Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant Human Deformed Epidermal Autoregulatory Factor 1 protein
Purity> 90 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMASMTGGQQMGRGHHHHHHENLYFQGEDSDSAAKQLGLAEAAAVAAAAAV AAAAAAAAGGEAEEPVLSRDEDSEEDADSEAERETPRVTAVAVMAAEPGH MDMGAEALPGPDEAAAAAAFAEVTTVTVANVGAAADNVFTTSVANAASIS GHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQ SPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKNRLGSGGRG RCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPH AASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLL PATAATTFTVTPSGQITTSGALTFDRASTVEATAVISESPAQGDVFAGAT VQEASVQPPCRASHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPA LAVPPPTPTKAAPPALVNGLELSEPRSWLYLEEMVNSLLNTAQQLKTLFE QAKHASTYREAATNQAKIHADAERKEQSCVNCGREAMSECTGCHKVNYCS TFCQRKDWKDHQHICGQSAAVTVQADEVHVAESVMEKVTV
Predicted molecular weight62 kDa including tags
Amino acids2 to 565
Additional sequence informationwith a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal.
- Anti-Deformed Epidermal Autoregulatory Factor 1 antibody [EPR10322] (ab156591)
- Anti-TEV Tag antibody [KT88] (ab179875)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
- Anti-T7 tag® antibody (ab9115)
- Anti-T7 tag® antibody (ab9138)
Our Abpromise guarantee covers the use of ab204219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, arginine, DTT and glycerol.
- DEAF1 transcription factor
FunctionTranscription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Required for neural tube closure and skeletal patterning. Regulates epithelial cell proliferation and side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 and retaining it in the cytoplasm.
Tissue specificityExpressed in various tissues and cells such as in peripheral mononuclear cells and hormone-secreting pituitary cells. Expression in pancreatic lymph nodes of patients with type 1 diabetes is 20 times higher than in healthy controls.
Sequence similaritiesContains 1 MYND-type zinc finger.
Contains 1 SAND domain.
Cellular localizationCytoplasm. Nucleus. When expressed alone, localizes mainly in the cytoplasm but, when expressed with isoform 1, nuclear localization is enhanced; Nucleus. Cytoplasm. Cytoplasmic in non-mucinous colorectal carcinoma. When expressed alone, localized almost exclusively in the nucleus but, when expressed with isoform 4, nuclear expression decreases to 32% and cytoplasmic expression increases by 270% and Secreted. Secreted in some cell types.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab204219 has not yet been referenced specifically in any publications.