Recombinant Human Deformed Epidermal Autoregulatory Factor 1 protein (ab204219)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant Human Deformed Epidermal Autoregulatory Factor 1 protein

  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    O75398

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MASMTGGQQMGRGHHHHHHENLYFQGEDSDSAAKQLGLAEAAAVAAAAAV AAAAAAAAGGEAEEPVLSRDEDSEEDADSEAERETPRVTAVAVMAAEPGH MDMGAEALPGPDEAAAAAAFAEVTTVTVANVGAAADNVFTTSVANAASIS GHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQ SPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKNRLGSGGRG RCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPH AASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLL PATAATTFTVTPSGQITTSGALTFDRASTVEATAVISESPAQGDVFAGAT VQEASVQPPCRASHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPA LAVPPPTPTKAAPPALVNGLELSEPRSWLYLEEMVNSLLNTAQQLKTLFE QAKHASTYREAATNQAKIHADAERKEQSCVNCGREAMSECTGCHKVNYCS TFCQRKDWKDHQHICGQSAAVTVQADEVHVAESVMEKVTV

    • Predicted molecular weight

      62 kDa including tags

    • Amino acids

      2 to 565

    • Additional sequence information

      with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal.

Specifications

Our Abpromise guarantee covers the use of ab204219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituent: 0.32% Tris HCl

    Contains NaCl, KCl, EDTA, arginine, DTT and glycerol.

General Info

  • Alternative names

    • Deaf1
    • DEAF1 transcription factor
    • DEAF1_HUMAN
    • Deformed epidermal autoregulatory factor 1 homolog
    • Nuclear DEA1 related transcriptional regulator
    • Nuclear DEAF-1-related transcriptional regulator
    • NUDR
    • SPN
    • Suppressin
    • Zinc finger MYND domain containing protein 5
    • Zinc finger MYND domain-containing protein 5
    • ZMYND5
    see all

  • Function

    Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Required for neural tube closure and skeletal patterning. Regulates epithelial cell proliferation and side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 and retaining it in the cytoplasm.

  • Tissue specificity

    Expressed in various tissues and cells such as in peripheral mononuclear cells and hormone-secreting pituitary cells. Expression in pancreatic lymph nodes of patients with type 1 diabetes is 20 times higher than in healthy controls.

  • Sequence similarities

    Contains 1 MYND-type zinc finger.
    Contains 1 SAND domain.

  • Cellular localization

    Cytoplasm. Nucleus. When expressed alone, localizes mainly in the cytoplasm but, when expressed with isoform 1, nuclear localization is enhanced; Nucleus. Cytoplasm. Cytoplasmic in non-mucinous colorectal carcinoma. When expressed alone, localized almost exclusively in the nucleus but, when expressed with isoform 4, nuclear expression decreases to 32% and cytoplasmic expression increases by 270% and Secreted. Secreted in some cell types.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab204219? Please let us know so that we can cite the reference in this datasheet.

ab204219 has not yet been referenced specifically in any publications.

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