Recombinant human DEP1 protein (ab42588)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Phosphatase Activity, Inhibition Assay
Description
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Product name
Recombinant human DEP1 protein
See all DEP1 proteins and peptides -
Biological activity
Specific Activity: 268 nmole/min/µg. Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and DEP1. Assay was done at 30&Deg;C for 10 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1
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Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
59 kDa -
Amino acids
1024 to 1306 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab42588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Phosphatase Activity
Inhibition Assay
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.307% Glutathione, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 0.0584% EDTA, 50% Glycerol (glycerin, glycerine), 0.435% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CD 148
- CD148
- CD148 antigen
see all -
Function
Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling. -
Tissue specificity
Expressed in the promyelocytic cell line HL60, the granulocyte-macrophage colony-stimulating factor-dependent leukemic cell line F-36P, and the interleukin-3 and erythropoietin-dependent leukemic cell line F-36E. Expressed predominantly in epithelial cells and lymphocytes. Enhanced expression at high cell density. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.
Contains 9 fibronectin type-III domains.
Contains 1 tyrosine-protein phosphatase domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane. Cell projection > ruffle membrane. Cell junction. After T cell stimulation, it is temporarily excluded from immunological synapses. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab42588 has not yet been referenced specifically in any publications.
