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    recombinant-human-dep1-protein-ab42588.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Phosphatases
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Recombinant human DEP1 protein (ab42588)

  • Datasheet
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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Phosphatase Activity, Inhibition Assay

Description

  • Product name

    Recombinant human DEP1 protein
    See all DEP1 proteins and peptides
  • Biological activity

    Specific Activity: 268 nmole/min/µg. Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and DEP1. Assay was done at 30&Deg;C for 10 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

    Q12913
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      59 kDa
    • Amino acids

      1024 to 1306
    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab42588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Phosphatase Activity

    Inhibition Assay

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.307% Glutathione, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 0.0584% EDTA, 50% Glycerol (glycerin, glycerine), 0.435% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CD 148
    • CD148
    • CD148 antigen
    • Density enhanced phosphatase 1
    • Density-enhanced phosphatase 1
    • DEP 1
    • DEP-1
    • HPTP eta
    • HPTPeta
    • Human density enhanced phosphatase 1
    • Protein tyrosine phosphatase eta
    • Protein tyrosine phosphatase receptor type J
    • Protein tyrosine phosphatase receptor type J polypeptide
    • Protein-tyrosine phosphatase eta
    • Protein-tyrosine phosphatase receptor type J
    • PTPJ
    • Ptprj
    • PTPRJ_HUMAN
    • R PTP Eta
    • R-PTP-eta
    • R-PTP-J
    • Receptor type tyrosine protein phosphatase eta
    • Receptor-type tyrosine-protein phosphatase eta
    • SCC 1
    • SCC1
    • susceptibility to colon cancer 1, mouse, homolog of
    see all
  • Function

    Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.
  • Tissue specificity

    Expressed in the promyelocytic cell line HL60, the granulocyte-macrophage colony-stimulating factor-dependent leukemic cell line F-36P, and the interleukin-3 and erythropoietin-dependent leukemic cell line F-36E. Expressed predominantly in epithelial cells and lymphocytes. Enhanced expression at high cell density.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.
    Contains 9 fibronectin type-III domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Cell membrane. Cell projection > ruffle membrane. Cell junction. After T cell stimulation, it is temporarily excluded from immunological synapses.
  • Target information above from: UniProt accession Q12913 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab42588? Please let us know so that we can cite the reference in this datasheet.

    ab42588 has not yet been referenced specifically in any publications.

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