Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human DEP1 protein (Active)
See all DEP1 proteins and peptides
The specific activity was determined to be approximately 95,000 nmol phosphate released /min/mg in a phosphatase assay using pNPP substrate.
Purity> 95 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Molecular weight informationSDS-PAGE molecular weight: ~65Kda.
Amino acids36 to 1337
TagsGST tag N-Terminus
Additional sequence informationGenBank: NM_002843
Our Abpromise guarantee covers the use of ab268909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.87% Sodium chloride, 0.31% Glutathione, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine), 0.42% MOPS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- CD 148
- CD148 antigen
FunctionTyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.
Tissue specificityExpressed in the promyelocytic cell line HL60, the granulocyte-macrophage colony-stimulating factor-dependent leukemic cell line F-36P, and the interleukin-3 and erythropoietin-dependent leukemic cell line F-36E. Expressed predominantly in epithelial cells and lymphocytes. Enhanced expression at high cell density.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.
Contains 9 fibronectin type-III domains.
Contains 1 tyrosine-protein phosphatase domain.
modificationsN- and O-glycosylated.
Cellular localizationCell membrane. Cell projection > ruffle membrane. Cell junction. After T cell stimulation, it is temporarily excluded from immunological synapses.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268909 has not yet been referenced specifically in any publications.