Recombinant Human Desmoplakin protein (His tag) (ab225667)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
-
Product name
Recombinant Human Desmoplakin protein (His tag) -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
CSDCLMRAELIVQPELKYGDGIQLTRSRELDECFAQANDQMEILDSLIRE MRQMGQPCDAYQKRLLQLQEQMRALYKAISVPRVRRASSKGGGGYTCQSG SGWDEFTKHVTSECLGWMRQQRAEMDMVAWGVDLASVEQHINSHRGIHNS IGDYRWQLDKIKADLREKSAIYQLEEEYENLLKASFERMDHLRQLQNIIQ ATSREIMWINDCEEEELLYDWSD -
Predicted molecular weight
42 kDa including tags -
Amino acids
78 to 300 -
Tags
His tag N-Terminus -
Additional sequence information
6xHis-SUMO tag at the N-terminus.
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab225667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Form
Liquid -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: 50% Glycerol (glycerin, glycerine), Tris buffer
General Info
-
Alternative names
- 250/210 kDa paraneoplastic pemphigus antigen
- Desmoplakin I
- Desmoplakin II
see all -
Relevance
Desmosomes are the most common type of intercellular junction in vertebrate epithelial cells. They are characterized by 2 forms of interaction with other cellular structures. First, they form membrane anchorage sites for intermediate-size filaments, which are seen as electron-dense plaques evident beneath the plasma membrane. Second, a specific membrane core domain interacts with a corresponding domain of the plasma membrane of an adjacent cell, apparently mediating intercellular adhesion in a stable way. The desmosome intermediate filament complex is thought to impart tensile strength and resilience to the epithelium. Desmosomal proteins can be divided into 2 groups based on whether they fractionate with the urea-insoluble 'core' or the urea-soluble 'plaque' components. Desmoglein is, for example, a protein of the core. The main proteins of the plaque comprise the desmoplakins and plakoglobin. -
Cellular localization
Cell junction, desmosome. Cytoplasm, cytoskeleton.
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab225667 has not yet been referenced specifically in any publications.