Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human DGKB protein (Active)
The specific activity of ab268442 was 3.9 nmol/min/mg in a kinase assay using Dilauroyl-sn-glycerol as substrate.
Purity> 90 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Molecular weight informationSDS-PAGE molecular weight: ~118kDa
TagsGST tag N-Terminus
Additional sequence informationGenBank: NM_004080
Our Abpromise guarantee covers the use of ab268442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 90 kDa diacylglycerol kinase
- DAG kinase beta
FunctionExhibits high phosphorylation activity for long-chain diacylglycerols.
Sequence similaritiesBelongs to the eukaryotic diacylglycerol kinase family.
Contains 1 DAGKc domain.
Contains 2 EF-hand domains.
Contains 2 phorbol-ester/DAG-type zinc fingers.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268442 has not yet been referenced specifically in any publications.