Description

  • Product name

    Recombinant Human DHODH protein
    See all DHODH proteins and peptides
  • Purity

    > 90 % SDS-PAGE.
    ab128451 is purified using conventional chromatography techniques (anion exchange and gel filtration)
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMTGDER FYAEHLMPTL QGLLDPESAH RLAVRFTSLG LLPRARFQDS DMLEVRVLGH KFRNPVGIAA GFDKHGEAVD GLYKMGFGFV EIGSVTPKPQ EGNPRPRVFR LPEDQAVINR YGFNSHGLSV VEHRLRARQQ KQAKLTEDGL PLGVNLGKNK TSVDAAEDYA EGVRVLGPLA DYLVVNVSSP NTAGLRSLQG KAELRRLLTK VLQERDGLRR VHRPAVLVKI APDLTSQDKE DIASVVKELG IDGLIVTNTT VSRPAGLQGA LRSETGGLSG KPLRDLSTQT IREMYALTQG RVPIIGVGGV SSGQDALEKI RAGASLVQLY TALTFWGPPV VGKVKRELEA LLKEQGFGGV TDAIGADHRR
    • Predicted molecular weight

      42 kDa including tags
    • Amino acids

      31 to 395
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab128451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Additional notes

    Not currently tested for endotoxin levels
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names

    • DHOdehase
    • Dhodh
    • Dihydroorotate dehydrogenase
    • Dihydroorotate dehydrogenase (quinone)
    • Dihydroorotate dehydrogenase mitochondrial
    • Dihydroorotate oxidase
    • Human complement of yeast URA1
    • mitochondrial
    • POADS
    • PYRD_HUMAN
    • URA1
    see all
  • Function

    Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
  • Pathway

    Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
  • Involvement in disease

    Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.
  • Sequence similarities

    Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
  • Post-translational
    modifications

    The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab128451 at approximately 42.3 kDa (3µg).

References

ab128451 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab128451.
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