Recombinant Human DHODH protein (His tag) (ab235065)

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Description

  • Product name

    Recombinant Human DHODH protein (His tag)
    See all DHODH proteins and peptides

  • Purity

    > 85 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    Q02127

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      TGDERFYAEHLMPTLQGLLDPESAHRLAVRFTSLGLLPRARFQDSDMLEV RVLGHKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPR PRVFRLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVN LGKNKTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELR RLLTKVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLI VTNTTVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPII GVGGVSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQ GFGGVTDAIGADHRR

    • Predicted molecular weight

      45 kDa including tags

    • Amino acids

      31 to 395

    • Tags

      His tag N-Terminus

    • Additional sequence information

      N-terminal 10xHis-tagged. Mitochondrial intermembrane.

Specifications

Our Abpromise guarantee covers the use of ab235065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Constituents: Tris buffer, 50% Glycerol

General Info

  • Alternative names

    • DHOdehase
    • Dhodh
    • Dihydroorotate dehydrogenase
    • Dihydroorotate dehydrogenase (quinone)
    • Dihydroorotate dehydrogenase mitochondrial
    • Dihydroorotate oxidase
    • Human complement of yeast URA1
    • mitochondrial
    • POADS
    • PYRD_HUMAN
    • URA1
    see all

  • Function

    Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.

  • Pathway

    Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.

  • Involvement in disease

    Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.

  • Sequence similarities

    Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

  • Post-translational
    modifications

    The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human DHODH protein (His tag) (ab235065)
    SDS-PAGE - Recombinant Human DHODH protein (His tag) (ab235065)

    ab235065 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

References

ab235065 has not yet been referenced specifically in any publications.

Publishing research using ab235065? Please let us know so that we can cite the reference in this datasheet.

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