Recombinant Human Di-ubiquitin (linkage-specific K27) (mutated L73 P) protein (ab207957)

Overview

  • Product name

    Recombinant Human Di-ubiquitin (linkage-specific K27) (mutated L73 P) protein
  • Protein length

    Full length protein

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL EDGRTLSDYNIQKESTLHLVLRPRGG
    • Amino acids

      1 to 76
    • Modifications

      mutated L73 P
    • Tags

      His tag N-Terminus
    • Additional sequence information

      6xHis-Non-cleavable K63 Poly-ubiquitin chain (2-4) are made using a 6xHis-Ubiquitin (L73P) mutant.

Specifications

Our Abpromise guarantee covers the use of ab207957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity

    95 % SDS-PAGE.

  • Form

    Liquid
  • Additional notes

    Ubiquitin chains, especially K63-linked ones, often form soluble aggregates even storing at -80°C. If necessary, urea powder can be added into the stock solution up to 3 M, then keep the stock solution at room temperature for 30 minutes. This treatment has no effect on Ubiquitin chain structure, but breaks soluble Ubiquitin chain aggregates.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Constituents: 0.24% Tris, 0.87% Sodium chloride, 0.02% Beta mercaptoethanol, 10% Glycerol

General Info

  • Relevance

    Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling
  • Cellular localization

    Cytoplasmic Nuclear

References

ab207957 has not yet been referenced specifically in any publications.

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