Recombinant Human DIAPH3 protein (ab153533)

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Overview

  • Product name

    Recombinant Human DIAPH3 protein

  • Protein length

    Protein fragment

Description

  • Nature

    Recombinant

  • Source

    Wheat germ
  • Amino Acid Sequence

    • Species

      Human

    • Sequence

      PDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPED LHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKKV

    • Amino acids

      632 to 729

    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab153533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form

    Liquid

  • Additional notes

    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • AN
    • AUNA1
    • Dia2
    • diap3
    • DIAP3_HUMAN
    • DIAPH3
    • Diaphanous homolog 3
    • Diaphanous homolog 3 (Drosophila)
    • Diaphanous related formin 3
    • Diaphanous, Drosophila, homolog of, 3
    • Diaphanous-related formin-3
    • DKFZp434C0931
    • DKFZp686A13178
    • DRF3
    • FLJ34705
    • mDia2
    • NSDAN
    • OTTHUMP00000018480
    • Protein diaphanous homolog 3
    • RP11-26P21.1
    see all

  • Function

    Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics.

  • Sequence similarities

    Belongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.

  • Domain

    DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human DIAPH3 protein (ab153533)
    SDS-PAGE - Recombinant Human DIAPH3 protein (ab153533)
    ab153533 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab153533 has not yet been referenced specifically in any publications.

Publishing research using ab153533? Please let us know so that we can cite the reference in this datasheet.

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