Bioactive grade

Recombinant human Dihydrofolate reductase (DHFR) protein (ab167972)

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SDS-PAGE - Recombinant human Dihydrofolate reductase (DHFR) protein (ab167972)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, Functional Studies

    Description

    • Product name

      Recombinant human Dihydrofolate reductase (DHFR) protein
      See all Dihydrofolate reductase (DHFR) proteins and peptides

    • Biological activity

      Specific activity is 1.5 - 2.5 units/ml and was obtained by measuring the oxidation of NADPH in absorbance at 340 nm during reaction. One unit will convert 1.0 µmole of 7, 8 dihydrofolate and beta-NADPH to 5, 6, 7, 8-tetrahydrofolate and beta-NADP per minute at pH 6.5 at 25°C.

    • Purity

      > 95 % SDS-PAGE.

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      Escherichia coli

    • Accession

      P00374

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMVGSLNCIVAVSQNMGIGKNGDLPWPPLRN EFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSREL KEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPG HLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKF EVYEKND

      • Predicted molecular weight

        24 kDa

      • Amino acids

        1 to 187

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab167972 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.03% DTT, 0.32% Tris HCl, 30% Glycerol, 0.58% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • DHFR
      • DHFRP1
      • Dihydrofolate reductase
      • DYR
      • DYR_HUMAN
      • EC 1.5.1.3
      see all

    • Function

      Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

    • Pathway

      Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

    • Sequence similarities

      Belongs to the dihydrofolate reductase family.
      Contains 1 DHFR (dihydrofolate reductase) domain.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human Dihydrofolate reductase (DHFR) protein (ab167972)
      SDS-PAGE - Recombinant human Dihydrofolate reductase (DHFR) protein (ab167972)
      15% SDS PAGE analysis of ab167972 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab167972? Please let us know so that we can cite the reference in this datasheet.

    ab167972 has not yet been referenced specifically in any publications.

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