Bioactive grade

Recombinant human Dihydrofolate reductase (DHFR) protein (ab167972)

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Description

  • Product name

    Recombinant human Dihydrofolate reductase (DHFR) protein
    See all Dihydrofolate reductase (DHFR) proteins and peptides

  • Biological activity

    Specific activity is 1.5 - 2.5 units/ml and was obtained by measuring the oxidation of NADPH in absorbance at 340 nm during reaction. One unit will convert 1.0 µmole of 7, 8 dihydrofolate and beta-NADPH to 5, 6, 7, 8-tetrahydrofolate and beta-NADP per minute at pH 6.5 at 25°C.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P00374

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMVGSLNCIVAVSQNMGIGKNGDLPWPPLRN EFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSREL KEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPG HLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKF EVYEKND

    • Predicted molecular weight

      24 kDa

    • Amino acids

      1 to 187

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab167972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.03% DTT, 0.32% Tris HCl, 30% Glycerol, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • DHFR
    • DHFRP1
    • Dihydrofolate reductase
    • DYR
    • DYR_HUMAN
    • EC 1.5.1.3
    see all

  • Function

    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

  • Pathway

    Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

  • Sequence similarities

    Belongs to the dihydrofolate reductase family.
    Contains 1 DHFR (dihydrofolate reductase) domain.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human Dihydrofolate reductase (DHFR) protein (ab167972)
    SDS-PAGE - Recombinant human Dihydrofolate reductase (DHFR) protein (ab167972)
    15% SDS PAGE analysis of ab167972 (3µg).

References

ab167972 has not yet been referenced specifically in any publications.

Publishing research using ab167972? Please let us know so that we can cite the reference in this datasheet.

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