Recombinant human Dihydrofolate reductase (DHFR) protein (ab167972)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human Dihydrofolate reductase (DHFR) protein
See all Dihydrofolate reductase (DHFR) proteins and peptides -
Biological activity
Specific activity is 1.5 - 2.5 units/ml and was obtained by measuring the oxidation of NADPH in absorbance at 340 nm during reaction. One unit will convert 1.0 µmole of 7, 8 dihydrofolate and beta-NADPH to 5, 6, 7, 8-tetrahydrofolate and beta-NADP per minute at pH 6.5 at 25°C. -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMVGSLNCIVAVSQNMGIGKNGDLPWPPLRN EFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSREL KEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPG HLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKF EVYEKND -
Predicted molecular weight
24 kDa -
Amino acids
1 to 187 -
Tags
His tag N-Terminus
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Associated products
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Related Products
- Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)
- Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)
- Anti-Dihydrofolate reductase (DHFR) antibody (ab152159)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Dihydrofolate reductase (DHFR) antibody (ab49881)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Dihydrofolate reductase (DHFR) antibody (ab82171)
- Anti-6X His tag® antibody (ab9108)
Specifications
Our Abpromise guarantee covers the use of ab167972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.03% DTT, 0.32% Tris HCl, 30% Glycerol, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- DHFR
- DHFRP1
- Dihydrofolate reductase
see all -
Function
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. -
Pathway
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1. -
Sequence similarities
Belongs to the dihydrofolate reductase family.
Contains 1 DHFR (dihydrofolate reductase) domain. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab167972 has not yet been referenced specifically in any publications.