Recombinant human Dihydrofolate reductase (DHFR) protein (ab87755)

Description

  • Product name

    Recombinant human Dihydrofolate reductase (DHFR) protein
    See all Dihydrofolate reductase (DHFR) proteins and peptides
  • Biological activity

    Specific activity is > 3500 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25oC.

  • Purity

    > 95 % SDS-PAGE.
    purified by using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      24 kDa
    • Amino acids

      1 to 187
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab87755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.0308% DTT, 0.316% Tris HCl, 30% Glycerol, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • DHFR
    • DHFRP1
    • Dihydrofolate reductase
    • DYR
    • DYR_HUMAN
    • EC 1.5.1.3
    see all
  • Function

    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
  • Pathway

    Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
  • Sequence similarities

    Belongs to the dihydrofolate reductase family.
    Contains 1 DHFR (dihydrofolate reductase) domain.
  • Information by UniProt

Images

  • ab87755 (lane 2) at 3µg visualized by 15% SDS-PAGE. Observed band size is approximately 27 kDa. (lane 1 MW marker).

References

ab87755 has not yet been referenced specifically in any publications.

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