Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human Dihydrofolate reductase (DHFR) protein
See all Dihydrofolate reductase (DHFR) proteins and peptides
Specific activity is > 3500 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25oC.
Purity> 95 % SDS-PAGE.
purified by using conventional chromatography techniques.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMGSSHHHHHH SSGLVPRGSH MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNLVIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPGHLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND
Predicted molecular weight24 kDa
Amino acids1 to 187
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab87755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.0308% DTT, 0.316% Tris HCl, 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Dihydrofolate reductase
FunctionKey enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
PathwayCofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Sequence similaritiesBelongs to the dihydrofolate reductase family.
Contains 1 DHFR (dihydrofolate reductase) domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab87755 has not yet been referenced specifically in any publications.