The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Measured by the ability to inhibit Wnt3-a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is typically 0.01-0.12 µg/ml in the presence of 10 ng/ml of rmWnt3a.
< 1.000 Eu/µg
>95% by SDS-PAGE .
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Constituents: 95% PBS, 5% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended to reconstitute the lyophilized protein in sterile water to a final concentration of 200 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Dickkopf 1 homolog
Dickkopf 1 like
Dickkopf homolog 1
Dickkopf like protein 1
Dickkopf related protein 1
Dickkopf WNT signaling pathway inhibitor 1
Dickkopf-related protein 1
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Belongs to the dickkopf family.
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.