Recombinant human DKK1 protein (ab155623)

Our Abpromise guarantee covers the use of ab155623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Biological activity

Measured by the ability to inhibit Wnt3-a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
The ED₅₀ for this effect is typically 0.01-0.12 µg/ml in the presence of 10 ng/ml of rmWnt3a.
Applications

Functional Studies

SDS-PAGE
Endotoxin level

< 1.000 Eu/µg
Purity

>95% by SDS-PAGE .
Form

Lyophilised
Concentration information loading...

Stability and Storage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

pH: 7.40
Constituents: 95% PBS, 5% Trehalose

This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitution

It is recommended to reconstitute the lyophilized protein in sterile water to a final concentration of 200 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

Alternative names
- Dickkopf 1
- Dickkopf 1 homolog
- Dickkopf 1 like
- Dickkopf homolog 1
- Dickkopf like protein 1
- Dickkopf related protein 1
- Dickkopf WNT signaling pathway inhibitor 1
- Dickkopf-1
- Dickkopf-related protein 1
- DKK 1
- Dkk-1
- Dkk1
- DKK1_HUMAN
- hDkk 1
- hDkk-1
- SK
see all
Function

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Tissue specificity

Placenta.
Sequence similarities

Belongs to the dickkopf family.
Domain

The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
Cellular localization

Secreted.
Target information above from: UniProt accession O94907 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

SDS-PAGE - Recombinant human DKK1 protein (ab155623)

SDS-PAGE analysis of reduced ab155623 stained overnight with Coomassie Blue.

ab155623 has not yet been referenced specifically in any publications.

Publishing research using ab155623? Please let us know so that we can cite the reference in this datasheet.

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