Bioactive grade

Recombinant human DKK1 protein (ab155623)

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Description

  • Product name

    Recombinant human DKK1 protein
    See all DKK1 proteins and peptides

  • Biological activity

    Measured by the ability to inhibit Wnt3-a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.

    The ED50 for this effect is typically 0.01-0.12 µg/ml in the presence of 10 ng/ml of rmWnt3a.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    O94907

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      TLNSVLNSNAIKNLPPPLGGAAGHPGSAVSAAPGILYPGGNKYQTIDNYQ PYPCAEDEECGTDEYCASPTRGGDAGVQICLACRKRRKRCMRHAMCCPGN YCKNGICVSSDQNHFRGEIEETITESFGNDHSTLDGYSRRTTLSSKMYHT KGQEGSVCLRSSDCASGLCCARHFWSKICKPVLKEGQVCTKHRRKGSHGL EIFQRCYCGEGLSCRIQKDHHQASNSSRLHTCQRH

    • Predicted molecular weight

      26 kDa including tags

    • Amino acids

      32 to 266

    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab155623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 95% PBS, 5% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile water to a final concentration of 200 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • Dickkopf 1
    • Dickkopf 1 homolog
    • Dickkopf 1 like
    • Dickkopf homolog 1
    • Dickkopf like protein 1
    • Dickkopf related protein 1
    • Dickkopf WNT signaling pathway inhibitor 1
    • Dickkopf-1
    • Dickkopf-related protein 1
    • DKK 1
    • Dkk-1
    • Dkk1
    • DKK1_HUMAN
    • hDkk 1
    • hDkk-1
    • SK
    see all

  • Function

    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

  • Tissue specificity

    Placenta.

  • Sequence similarities

    Belongs to the dickkopf family.

  • Domain

    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human DKK1 protein (ab155623)
    SDS-PAGE - Recombinant human DKK1 protein (ab155623)
    Human Dkk-1 (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
  • Functional Studies - Recombinant human DKK1 protein (ab155623)
    Functional Studies - Recombinant human DKK1 protein (ab155623)

    Immobilized Hab155623 at 5 μg/mL (100 μL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag with a linear range of 0.01-0.156 μg/mL

  • SDS-PAGE - Recombinant human DKK1 protein (ab155623)
    SDS-PAGE - Recombinant human DKK1 protein (ab155623)
    SDS-PAGE analysis of reduced ab155623 stained overnight with Coomassie Blue.

References

ab155623 has not yet been referenced specifically in any publications.

Publishing research using ab155623? Please let us know so that we can cite the reference in this datasheet.

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