Description

  • Product name

    Recombinant human DLL4 protein
    See all DLL4 proteins and peptides
  • Biological activity

    Biological Activity : ab84081, when immobilized at concentrations >1.5 µg/mL will inhibit myogenesis in C2C12 cells.
  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.
  • Endotoxin level

    < 0.100 Eu/µg
  • Expression system

    HEK 293 cells
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      SGVFQLQLQE FINERGVLAS GRPCEPGCRT FFRVCLKHFQ AVVSPGPCTF GTVSTPVLGT NSFAVRDDSS GGGRNPLQLP FNFTWPGTFS LIIEAWHAPG DDLRPEALPP DALISKIAIQ GSLAVGQNWL LDEQTSTLTR LRYSYRVICS DNYYGDNCSR LCKKRNDHFG HYVCQPDGNL SCLPGWTGEY CQQPICLSGC HEQNGYCSKP AECLCRPGWQ GRLCNECIPH NGCRHGTCST PWQCTCDEGW GGLFCDQDLN YCTHHSPCKN GATCSNSGQR SYTCTCRPGY TGVDCELELS ECDSNPCRNG GSCKDQEDGY HCLCPPGYYG LHCEHSTLSC ADSPCFNGGS CRERNQGANY ACECPPNFTG SNCEKKVDRC TSNPCANGGQ CLNRGPSRMC RCRPGFTGTY CELHVSDCAR NPCAHGGTCH DLENGLMCTC PAGFSGRRCE VRTSIDACAS SPCFNRATCY TDLSTDTFVC NCPYGFVGSR CEFPVGLP
    • Amino acids

      27 to 524

Associated products

Specifications

Our Abpromise guarantee covers the use of ab84081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstituted ab84081 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20oC. Avoid repeated freeze/thaw cycles.

General Info

  • Alternative names

    • AOS6
    • Delta 4
    • delta 4 precursor
    • Delta ligand 4
    • delta ligand 4 precursor
    • Delta like 4
    • Delta like 4 homolog
    • Delta like 4 protein
    • Delta like canonical Notch ligand 4
    • Delta like protein 4
    • Delta-like 4 (Drosophila)
    • Delta-like protein 4
    • Delta4
    • DLL 4
    • Dll4
    • DLL4_HUMAN
    • Drosophila Delta homolog 4
    • hdelta2
    • Homeobox protein DLL-4
    • MGC126344
    • Notch ligand delta 2
    • Notch ligand DLL4
    • Notch ligand DLL4 precursor
    • XDLL-4
    see all
  • Function

    Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.
  • Tissue specificity

    Expressed in vascular endothelium.
  • Sequence similarities

    Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • Domain

    The Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
  • Post-translational
    modifications

    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localization

    Membrane.
  • Information by UniProt

References

This product has been referenced in:

  • Yu VW  et al. Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow. J Exp Med 212:759-74 (2015). Read more (PubMed: 25918341) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you very much for contacting us with your question.

I've looked over both of these products, and they are both active DLL4 proteins. I'm not sure why ab108557 does not have "active" in its name, but I will recommend that this is updated.

Ab108557 has been shown to inhibit adipogenesis of 3T3L-1 cells and MSC's and also to induce Hes-1 in 3T3L-1 cells.Ab84081 has been used to inhibit myogenesis in C2C12 cells.

The major difference between these proteins is that ab84081 corresponds to amino acids 27-524 of human DLL4, while ab108557 is amino acids 1-529 and it is fused to a human IgG1 Fc fragment at the C terminus.

I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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