The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
>90% by SDS-PAGE.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C.
Information available upon request.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
DinB homolog 1 (E. coli)
DNA damage inducible protein b
DNA polymerase kappa
Polymerase (DNA directed) kappa
polymerase, DNA, kappa
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
Belongs to the DNA polymerase type-Y family. Contains 2 UBZ-type zinc fingers. Contains 1 umuC domain.
The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
Nucleus. Detected throughout the nucleus and at replication foci.
Functional Studies - Recombinant human DNA Polymerase Kappa/POLK protein (ab176956)
DNA polymerizing activity of ab176956 50 mM Tris-HCl pH 8.0, 2 mM MgCl2, 1 mM DTT, 0.1 mg / ml BSA, 0.1 mM dNTP, 100 nM primer/template(13-mer/30-mer), DNA polymerase kappa/POLK at 13~50 ng/25μl (lanes 1,2, and 3 respectiviely) per assay, incubated at 37℃ for 10 min.
SDS-PAGE - Recombinant human DNA Polymerase Kappa/POLK protein (ab176956)