Key features and details
- Expression system: CHO cells
- Active: Yes
- Suitable for: WB, Functional Studies
Product nameRecombinant human DNase I protein (Active)
See all DNase I proteins and peptides
One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25°C when acting upon highly polymerized solution of DNA at pH-5. (Also, 0.005 Kunitz unit digests 1µg of lambda phage DNA in 10 minutes at 37°C in 50mM Tris, 1mM Mg2+, pH 7.8 in a 50ul reaction volume.)
Expression systemCHO cells
Protein lengthFull length protein
Predicted molecular weight37 kDa
Our Abpromise guarantee covers the use of ab73430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Three weeks at 15°C, pH-6.3.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C. Please see notes section.
Constituents: 0.015% Calcium chloride, 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Deoxyribonuclease 1
- Deoxyribonuclease I
FunctionAmong other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.
Tissue specificityPrincipally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.
Sequence similaritiesBelongs to the DNase I family.
Cellular localizationSecreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope.
- Information by UniProt
Anti-DNase I antibody (ab97393) at 1/1000 dilution +
Recombinant human DNase I protein (Active) (ab73430) at 0.001 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Exposure time: 4 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab73430 has not yet been referenced specifically in any publications.