Recombinant human DNase I protein (Active) (ab73430)

Description

  • Product name

    Recombinant human DNase I protein (Active)
  • Biological activity

    1000IU/1mg.

    One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25°C when acting upon highly polymerized solution of DNA at pH-5. (Also, 0.005 Kunitz unit digests 1µg of lambda phage DNA in 10 minutes at 37°C in 50mM Tris, 1mM Mg2+, pH 7.8 in a 50ul reaction volume.)

  • Expression system

    CHO cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      37 kDa

Specifications

Our Abpromise guarantee covers the use of ab73430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

  • Form

    Liquid
  • Additional notes

    Stability:
    2 years when stored at 4°C, three weeks at 15°C, pH-6.3.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    Constituents: 0.015% Calcium chloride, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Deoxyribonuclease 1
    • Deoxyribonuclease I
    • Deoxyribonuclease-1
    • Deoxyribonuclease1
    • DeoxyribonucleaseI
    • DNAS1_HUMAN
    • DNASE 1
    • DNase I
    • DNase I lysosomal
    • DNASE1
    • DNaseI
    • DNL 1
    • DNL1
    • Dornase alfa
    • DRNI
    • FLJ38093
    • Human urine deoxyribonuclease I
    see all
  • Function

    Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.
  • Tissue specificity

    Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.
  • Sequence similarities

    Belongs to the DNase I family.
  • Cellular localization

    Secreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope.
  • Information by UniProt

Images

References

ab73430 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

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Answer

There are no tags on this DNAse I protein.

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Answer

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