Recombinant human Dnmt1 protein (ab198109)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human Dnmt1 protein
See all Dnmt1 proteins and peptides -
Biological activity
Specific Activity: ≥0.16 pmol/min/µg.
Assay Conditions: Assay was performed using a DNMT1 Assay kit, using 40 µM S-adenosylmethionine for 1 hour at 37°C.
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Purity
> 95 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MHHHHHHDYKDDDDKEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSN SDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGD SDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKAT TTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKA CKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMH QGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDD SSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDE CEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWY DQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDS RVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLY PEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVN KFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLP ECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGK PKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEM WDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEM LCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVR NFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAA PGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRD TMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSA LVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSS GALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEW DGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNI LDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD -
Predicted molecular weight
130 kDa including tags -
Amino acids
501 to 1632 -
Tags
His-DDDDK tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)
80 ng/µl DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- ADCADN
- AIM
- CXXC finger protein 9
see all -
Function
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. -
Tissue specificity
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1. -
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 2 BAH domains.
Contains 1 CXXC-type zinc finger. -
Domain
The N-terminal part is required for homodimerization and acts as a regulatory domain. -
Post-translational
modificationsSumoylated; sumoylation increases activity. -
Cellular localization
Nucleus. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab198109 has not yet been referenced specifically in any publications.