Recombinant Human dNT-2 protein (ab113140)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant Human dNT-2 protein -
Purity
> 95 % SDS-PAGE.
ab113140 was purified using conventional chromatography. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGGRALRVLVDMDGVLADFEGGFLRKFRAR FPDQPFIALEDRRGFWVS EQYGRLRPGLSEKAISIWESKNFFFELEPL PGAVEAVKEMASLQNTDVFICTSPIKMFKYCPYEKYAWVEKYFGP DFL EQIVLTRDKTVVSADLLIDDRPDITGAEPTPSWEHVLFTACHNQHLQLQP PRRRLHSWADDWKAILDSKRPC -
Predicted molecular weight
25 kDa including tags -
Amino acids
32 to 228 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab113140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Additional notes
Previously labelled as NT5M.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
General Info
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Alternative names
- 3''-nucleotidase
- 5''
- 5''(3'')-deoxyribonucleotidase
see all -
Function
Dephosphorylates specifically the 5' and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides, and so protects mitochondrial DNA replication from excess dTTP. Has only marginal activity towards dIMP and dGMP. -
Tissue specificity
Highly expressed in heart, brain and skeletal muscle. Detected at very low levels in kidney and pancreas. -
Sequence similarities
Belongs to the 5'(3')-deoxyribonucleotidase family. -
Cellular localization
Mitochondrion. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab113140 has not yet been referenced specifically in any publications.