Recombinant Human Dopamine beta Hydroxylase protein (ab202616)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Dopamine beta Hydroxylase protein
See all Dopamine beta Hydroxylase proteins and peptides -
Purity
> 85 % SDS-PAGE.
ab202616 was purified by using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMSAPRESPLPYHIPLDPEGSLELSWNVSYT QEAIHFQLLVRRLKAGVLFGMSDRGELENADLVVLWTDGDTAYFADAWSD QKGQIHLDPQQDYQLLQVQRTPEGLTLLFKRPFGTCDPKDYLIEDGTVHL VYGILEEPFRSLEAINGSGLQMGLQRVQLLKPNIPEPELPSDACTMEVQA PNIQIPSQETTYWCYIKELPKGFSRHHIIKYEPIVTKGNEALVHHMEVFQ CAPEMDSVPHFSGPCDSKMKPDRLNYCRHVLAAWALGAKAFYYPEEAGLA FGGPGSSRYLRLEVHYHNPLVIEGRNDSSGIRLYYTAKLRRFNAGIMELG LVYTPVMAIPPRETAFILTGYCTDKCTQLALPPSGIHIFASQLHTHLTGR KVVTVLVRDGREWEIVNQDNHYSPHFQEIRMLKKVVSVHPGDVLITSCTY NTEDRELATVGGFGILEEMCVNYVHYYPQTQLELCKSAVDAGFLQKYFHL INRFNNEDVCTCPQASVSQQFTSVPWNSFNRDVLKALYSFAPISMHCNKS SAVRFQGEWNLQPLPKVISTLEEPTPQCPTSQGRSPAGPTVVSIGGGKG -
Predicted molecular weight
67 kDa including tags -
Amino acids
40 to 617 -
Tags
His tag N-Terminus -
Additional sequence information
NP_000778. Soluble Dopamine beta Hydroxylase.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab202616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 89% PBS, 10% Glycerol (glycerin, glycerine), 0.02% DTT
General Info
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Alternative names
- dbh
- DBM
- Dopamine beta hydroxylase
see all -
Function
Conversion of dopamine to noradrenaline. -
Pathway
Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1. -
Involvement in disease
Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction. -
Sequence similarities
Belongs to the copper type II ascorbate-dependent monooxygenase family.
Contains 1 DOMON domain. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab202616 has not yet been referenced specifically in any publications.