Recombinant Human Dopamine beta Hydroxylase protein (His tag) (ab276542)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Dopamine beta Hydroxylase protein (His tag)
See all Dopamine beta Hydroxylase proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
VAIFLVILVA ALQGSAPRES PLPYHIPLDP EGSLELSWNV SYTQEAIHFQ LLVRRLKAGV LFGMSDRGEL ENADLVVLWT DGDTAYFADA WSDQKGQIHL DPQQDYQLLQ VQRTPEGLTL LFKRPFGTCD PKDYLIEDGT VHLVYGILEE PFRSLEAING SGLQMGLQRV QLLKPNIPEP ELPSDACTME VQAPNIQIPS QETTYWCYIK ELPKGFSRHH IIKYEPIVTK GNEALVHHME VFQCAPEMDS VPHFSGPCDS KMKPDRLNYC RHVLAAWALG AKAFYYPEEA GLAFGGPGSS RYLRLEVHYH NPLVIEGRND SSGIRLYYTA KLRRFNAGIM ELGLVYTPVM AIPPRETAFI LTGYCTDKCT QLALPPSGIH IFASQLHTHL TGRKVVTVLV RDGREWEIVN QDNHYSPHFQ EIRMLKKVVS VHPGDVLITS CTYNTEDREL ATVGGFGILE EMCVNYVHYY PQTQLELCKS AVDAGFLQKY FHLINRFNNE DVCTCPQASV SQQFTSVPWN SFNRDVLKAL YSFAPISMHC NKSSAVRFQG EWNLQPLPKV ISTLEEPTPQ CPTSQGRS -
Predicted molecular weight
67 kDa including tags -
Molecular weight information
The recombinant human DBH comprises 598 amino acids and has a predicted molecular mass of 67.3 kDa. The apparent molecular mass of the protein is approximately 68 kDa in SDS-PAGE under reducing conditions due to glycosylation. -
Amino acids
26 to 603 -
Tags
His tag N-Terminus -
Additional sequence information
Predicted N-terminus: His.
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Specifications
Our Abpromise guarantee covers the use of ab276542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituent: 100% PBS -
ReconstitutionThis information is lot specific. Please contact our technical Support team for details.
General Info
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Alternative names
- dbh
- DBM
- Dopamine beta hydroxylase
see all -
Function
Conversion of dopamine to noradrenaline. -
Pathway
Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1. -
Involvement in disease
Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction. -
Sequence similarities
Belongs to the copper type II ascorbate-dependent monooxygenase family.
Contains 1 DOMON domain. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab276542 has not yet been referenced specifically in any publications.