Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human DRAK2 protein (Active)
See all DRAK2 proteins and peptides
Assay was done in kinase buffer containing 1 mM DTT using MRCL3 peptide substrate (0.2 mg/ml) and 20 μM ATP. Reaction was performed at 30°C for 50 mins. Amount of ATP transferred was calculated using Kinase-Glo reagent.
Purity>= 90 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceSRRRFDCRSISGLLTTTPQIPIKMENFNNFYILTSKELGRGKFAVVRQCI SKSTGQEYAAKFLKKRRRGQDCRAEILHEIAVLELAKSCPRVINLHEVYE NTSEIILILEYAAGGEIFSLCLPELAEMVSENDVIRLIKQILEGVYYLHQ NNIVHLDLKPQNILLSSIYPLGDIKIVDFGMSRKIGHACELREIMGTPEY LAPEILNYDPITTATDMWNIGIIAYMLLTHTSPFVGEDNQETYLNISQVN VDYSEETFSSVSQLATDFIQSLLVKNPEKRPTAEICLSHSWLQQWDFENL FHPEETSSSSQTQDHSVRSSEDKTSKSSCNGTCGDREDKENIPEDSSMVS KRFRFDDSLPNPHELVSDLLC
Predicted molecular weight43 kDa
Amino acids2 to 372
Our Abpromise guarantee covers the use of ab271489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.03% EDTA
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- DAP kinase related apoptosis inducing protein
- DAP kinase related apoptosis inducing protein kinase 2
- DAP kinase-related apoptosis-inducing protein kinase 2
FunctionActs as a positive regulator of apoptosis.
Tissue specificityHighly expressed in placenta, lung, pancreas. Lower levels in heart, brain, liver, skeletal muscle and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271489 has not yet been referenced specifically in any publications.