Overview

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      TKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVTASGYEGVLRH KGVRPELVGRLGRCGHMYHLLCLVAMYSNGNKDGSLQCPT
    • Amino acids
      381 to 470
    • Tags
      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab158327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 mg/ml.

    Previously labelled as Deltex.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • Deltex
    • Deltex 1, E3 ubiquitin ligase
    • Deltex homolog 1
    • Deltex homolog 1 (Drosophila)
    • Deltex protein 1
    • Deltex-1
    • Deltex1
    • dtx1
    • DTX1_HUMAN
    • E3 ubiquitin protein ligase DTX1
    • E3 ubiquitin-protein ligase DTX1
    • FXI-T1
    • Fxit 1
    • Fxit1
    • hDTX1
    • hDx 1
    • mDTX1
    • Protein deltex 1
    • Protein deltex-1
    see all
  • Function
    Functions as a ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1.
  • Tissue specificity
    Widely expressed. Strongly expressed in blood vessel. Also expressed in embryonic nervous system, pancreas, lung, adrenal gland, digestive tube and muscles. Expressed in MZB cells and developing B- and T-cells.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the Deltex family.
    Contains 1 RING-type zinc finger.
    Contains 2 WWE domains.
  • Domain
    The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.
  • Post-translational
    modifications
    Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
  • Cellular localization
    Cytoplasm. Nucleus. Predominantly cytoplasmic. Associates with endocytic vesicles. Partially nuclear.
  • Information by UniProt

Images

  • ab158327 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab158327 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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