Description

  • Product name

    Recombinant Human DTYMK protein
    See all DTYMK proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    No detergents such as urea, Triton or Tween have been used to purify the protein. Purified by using anion exchange chromatography (DEAE sepharose resin) and gel filtration chromatography (Sephacryl S 200) with 20mM Tris pH 7.5, 2mM EDTA.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MAARRGALIV LEGVDRAGKS TQSRKLVEAL CAAGHRAELL RFPERSTEIG KLLSSYLQKK SDVEDHSVHL LFSANRWEQV PLIKEKLSQG VTLVVDRYAF SGVAFTGAKE NFSLDWCKQP DVGLPKPDLV LFLQLQLADA AKRGAFGHER YENGAFQERA LRCFHQLMKD TTLNWKMVDA SKSIEAVHED IRVLSEDAIR TATEKPLGEL WK
    • Predicted molecular weight

      24 kDa
    • Amino acids

      1 to 212
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab109838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Additional notes


    Via its role in the catalytic creation of dTDP, DTYMK plays an important role in the pathway of DNA synthesis and is thought to be involved in cell cycle progression and cell growth.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 20% Glycerol, 0.04% DTT, 0.58% Sodium chloride

General Info

  • Alternative names

    • CDC8
    • deoxythymidylate kinase
    • Deoxythymidylate kinase (thymidylate kinase)
    • dTMP kinase
    • Dtymk
    • FLJ44192
    • KTHY_HUMAN
    • MGC198617
    • OTTHUMP00000164604
    • OTTHUMP00000200145
    • OTTHUMP00000200146
    • PP3731
    • Thymidylate kinase
    • TMPK
    • TYMK
    see all
  • Function

    Catalyzes the conversion of dTMP to dTDP.
  • Pathway

    Pyrimidine metabolism; dTTP biosynthesis.
  • Sequence similarities

    Belongs to the thymidylate kinase family.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab109838: (1) MW marker, (2) ab109838 at 3µg
    Gel concentration: 15%

References

This product has been referenced in:

  • Su JY & Sclafani RA Molecular cloning and expression of the human deoxythymidylate kinase gene in yeast. Nucleic Acids Res 19:823-7 (1991). Read more (PubMed: 2017365) »
  • Cummins RR & Balinsky D Activities of some enzymes of pyrimidine and DNA synthesis in a rat transplantable hepatoma and human primary hepatomas, in cell lines derived from these tissues, and in human fetal liver. Cancer Res 40:1235-9 (1980). Read more (PubMed: 6244089) »
See all 2 Publications for this product

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