Recombinant Human DUSP10 protein (ab131842)

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SDS-PAGE - Recombinant Human DUSP10 protein (ab131842)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human DUSP10 protein

    • Expression system

      Wheat germ

    • Accession

      Q9Y6W6

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MQRLNIGYVINVTTHLPLYHYEKGLFNYKRLPATDSNKQNLRQYFEEAFE FIEEAHQCGKGLLIHCQAGVSRSATIVIAYLMKHTRMTMTDAYKFVKGKR PIISPNLNFMGQLLEFEEDLNNGVTPRILTPKLMGVETVV

      • Predicted molecular weight

        41 kDa including tags

      • Amino acids

        1 to 140

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab131842 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      SDS-PAGE

      Western blot

    • Form

      Liquid

    • Additional notes

      Protein concentration is above or equal to 0.05 mg/mL.

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

      Glutathione is reduced.

    General Info

    • Alternative names

      • Dual specificity phosphatase 10
      • Dual specificity phosphatase MKP 5
      • Dual specificity phosphatase MKP5
      • Dual specificity protein phosphatase 10
      • DUS10_HUMAN
      • DUSP 10
      • DUSP10
      • DUSP10 protein
      • MAP kinase phosphatase 5
      • Mitogen activated protein kinase phosphatase 5
      • Mitogen-activated protein kinase phosphatase 5
      • MKP 5
      • MKP-5
      • MKP5
      • Serine/threonine specific protein phosphatase
      see all

    • Function

      Involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily.

    • Sequence similarities

      Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
      Contains 1 rhodanese domain.
      Contains 1 tyrosine-protein phosphatase domain.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human DUSP10 protein (ab131842)
      SDS-PAGE - Recombinant Human DUSP10 protein (ab131842)
      12.5% SDS-PAGE stained with Coomassie Blue showing ab13142 at approximately 41.14 kDa.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab131842? Please let us know so that we can cite the reference in this datasheet.

    ab131842 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Is this protein active?

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    Answer

    Thank you for contacting us. The activity for ab 113160 is as follows:

    Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate

    The activity of all the other proteins has not yet been tested. As a rule it should say "active" in the titale if it is active, however when new testing occurs it is not always immediately put into the title, so if there are ever any proteins you interested in we can always check them for you since once in a while you find one that has been tested but not updated. We currently have a project in the works that will update this active status more immediately.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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