Overview

  • Product name

    Recombinant Human DUSP18 protein
  • Protein length

    Full length protein

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMTAPSCAFPV QFRQPSVSGL SQITKSLYIS NGVAANNKLM LSSNQITMVI NVSVEVVNTL YEDIQYMQVP VADSPNSRLC DFFDPIADHI HSVEMKQGRT LLHCAAGVSR SAALCLAYLM KYHAMSLLDA HTWTKSCRPI IRPNSGFWEQ LIHYEFQLFG KNTVHMVSSP VGMIPDIYEK EVRLMIPL
    • Molecular weight

      24 kDa including tags
    • Amino acids

      1 to 188
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab123154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF
  • Purity

    > 95 % SDS-PAGE.
    ab123154 purified by using conventional chromatography techniques.
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.002% PMSF, 0.02% DTT, 0.32% Tris HCl, 0.03% EDTA, 40% Glycerol

General Info

  • Alternative names

    • Dual specificity protein phosphatase 18
    • DUS18_HUMAN
    • Dusp18
    • LMW-DSP20
    • LMWDSP20
    • Low molecular weight dual specificity phosphatase 20
    see all
  • Function

    Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. In vitro, dephosphorylates p-nitrophenyl phosphate (pNPP).
  • Tissue specificity

    Widely expressed with highest levels in liver, brain, ovary and testis.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab123154 (3 µg).

References

ab123154 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for contacting us. The activity for ab 113160 is as follows:

Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate

The activity of all the other proteins has not yet been tested. As a rule it should say "active" in the titale if it is active, however when new testing occurs it is not always immediately put into the title, so if there are ever any proteins you interested in we can always check them for you since once in a while you find one that has been tested but not updated. We currently have a project in the works that will update this active status more immediately.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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