Recombinant Human DUSP4 protein (ab132331)

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SDS-PAGE - Recombinant Human DUSP4 protein (ab132331)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: WB, SDS-PAGE, ELISA

    Description

    • Product name

      Recombinant Human DUSP4 protein

    • Expression system

      Wheat germ

    • Accession

      Q13115

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MVTMEELREMDCSVLKRLMNRDENGGGAGGSGSHGTLGLPSGGKCLLLDC RPFLAHSAGYILGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSG LYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERF SSEYPEFCSKTKALAAIPPPVPPSATEPLDLGCSSCGTPLHDQGGPVEIL PFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDN HKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKR VRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCAAEAASPSGPL RERGKTPATPTSQFVFSFPVSVGVHSAPSSLPYLHSPITTSPSC

      • Predicted molecular weight

        69 kDa including tags

      • Amino acids

        1 to 394

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab132331 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      SDS-PAGE

      ELISA

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • Dual specificity phosphatase 4
      • Dual specificity protein phosphatase 4
      • Dual specificity protein phosphatase hVH2
      • DUS4_HUMAN
      • DUSP 4
      • Dusp4
      • HVH 2
      • HVH2
      • MAP kinase phosphatase 2
      • Mitogen activated protein kinase phosphatase 2
      • Mitogen-activated protein kinase phosphatase 2
      • MKP 2
      • MKP-2
      • MKP2
      • Serine/threonine specific protein phosphatase
      • TYP
      • Typ1
      • VH 2
      • VH1 homologous phosphatase 2
      • VH2
      see all

    • Function

      Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.

    • Sequence similarities

      Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
      Contains 1 rhodanese domain.
      Contains 1 tyrosine-protein phosphatase domain.

    • Post-translational
      modifications

      Phosphorylation in the C-terminus by ERK1/2 inhibits proteasomal degradation and stabilizes the protein.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human DUSP4 protein (ab132331)
      SDS-PAGE - Recombinant Human DUSP4 protein (ab132331)
      12.5% SDS-PAGE analysis of ab132331 stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab132331? Please let us know so that we can cite the reference in this datasheet.

    ab132331 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Is this protein active?

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    Answer

    Thank you for contacting us. The activity for ab 113160 is as follows:

    Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate

    The activity of all the other proteins has not yet been tested. As a rule it should say "active" in the titale if it is active, however when new testing occurs it is not always immediately put into the title, so if there are ever any proteins you interested in we can always check them for you since once in a while you find one that has been tested but not updated. We currently have a project in the works that will update this active status more immediately.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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