Recombinant Human DUSP4 protein (ab132331)

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Description

  • Product name

    Recombinant Human DUSP4 protein

  • Expression system

    Wheat germ

  • Accession

    Q13115

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MVTMEELREMDCSVLKRLMNRDENGGGAGGSGSHGTLGLPSGGKCLLLDC RPFLAHSAGYILGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSG LYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERF SSEYPEFCSKTKALAAIPPPVPPSATEPLDLGCSSCGTPLHDQGGPVEIL PFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDN HKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKR VRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCAAEAASPSGPL RERGKTPATPTSQFVFSFPVSVGVHSAPSSLPYLHSPITTSPSC

    • Predicted molecular weight

      69 kDa including tags

    • Amino acids

      1 to 394

Associated products

Specifications

Our Abpromise guarantee covers the use of ab132331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    ELISA

  • Form

    Liquid

  • Additional notes

    This protein is best used within three months from the date of receipt.

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • Dual specificity phosphatase 4
    • Dual specificity protein phosphatase 4
    • Dual specificity protein phosphatase hVH2
    • DUS4_HUMAN
    • DUSP 4
    • Dusp4
    • HVH 2
    • HVH2
    • MAP kinase phosphatase 2
    • Mitogen activated protein kinase phosphatase 2
    • Mitogen-activated protein kinase phosphatase 2
    • MKP 2
    • MKP-2
    • MKP2
    • Serine/threonine specific protein phosphatase
    • TYP
    • Typ1
    • VH 2
    • VH1 homologous phosphatase 2
    • VH2
    see all

  • Function

    Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.

  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.

  • Post-translational
    modifications

    Phosphorylation in the C-terminus by ERK1/2 inhibits proteasomal degradation and stabilizes the protein.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human DUSP4 protein (ab132331)
    SDS-PAGE - Recombinant Human DUSP4 protein (ab132331)
    12.5% SDS-PAGE analysis of ab132331 stained with Coomassie Blue.

References

ab132331 has not yet been referenced specifically in any publications.

Publishing research using ab132331? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Is this protein active?

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Answer

Thank you for contacting us. The activity for ab 113160 is as follows:

Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate

The activity of all the other proteins has not yet been tested. As a rule it should say "active" in the titale if it is active, however when new testing occurs it is not always immediately put into the title, so if there are ever any proteins you interested in we can always check them for you since once in a while you find one that has been tested but not updated. We currently have a project in the works that will update this active status more immediately.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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