Description

  • Product name

    Recombinant human DYRK3 protein
  • Biological activity

    The specific activity of ab135222 was determined to be 242 nmol/min/mg.
  • Purity

    > 70 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MKVEQLFQEFGNRKSNTIQSDGISDSEKCSPTVSQGKSSDCLNTVKSNSS SKAPKVVPLTPEQALKQYKHHLTAYEKLEIINYPEIYFVGPNAKKRHGVI GGPNNGGYDDADGAYIHVPRDHLAYRYEVLKIIGKGSFGQVARVYDHKLR QYVALKMVRNEKRFHRQAAEEIRILEHLKKQDKTGSMNVIHMLESFTFRN HVCMAFELLSIDLYELIKKNKFQGFSVQLVRKFAQSILQSLDALHKNKII HCDLKPENILLKHHGRSSTKVIDFGSSCFEYQKLYTYIQSRFYRAPEIIL GSRYSTPIDIWSFGCILAELLTGQPLFPGEDEGDQLACMMELLGMPPPKL LEQSKRAKYFINSKGIPRYCSVTTQADGRVVLVGGRSRRGKKRGPPGSKD WGTALKGCDDYLFIEFLKRCLHWDPSARLTPAQALRHPWISKSVPRPLTT IDKVSGKRVVNPASAFQGLGSKLPPVVGIANKLKANLMSETNGSIPLCSV LPKLIS
    • Predicted molecular weight

      95 kDa including tags
    • Amino acids

      63 to 568

Associated products

Specifications

Our Abpromise guarantee covers the use of ab135222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Additional notes

    ab204857 (DYRKtide peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Dual specificity tyrosine (Y) phosphorylation regulated kinase 3
    • Dual specificity tyrosine phosphorylation-regulated kinase 3
    • Dual specificity tyrosine-phosphorylation-regulated kinase 3
    • Dyrk3
    • DYRK3_HUMAN
    • DYRK5
    • hYAK3 2
    • Protein kinase Dyrk3
    • RED
    • REDK
    • Regulatory erythroid kinase
    see all
  • Function

    Negative regulator of EPO-dependent erythropoiesis, may place an upper limit on red cell production during stress erythropoiesis. Inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells. May act by regulating CREB/CRE signaling.
  • Tissue specificity

    Isoform 1 and isoform 2 are highly expressed in testis and in hematopoietic tissue such as fetal liver, and bone marrow. Isoform 2 is the predominant form in testis. Isoform 1 is the predominant form in fetal liver and bone marrow. Isoform 1 and isoform 2 are present at low levels in heart, pancreas, lymph node, and thymus.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated on tyrosine residues.
  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • The specific activity of DYRK3 (ab135222) was determined to be 315 nmol/min/mg as per activity assay protocol and was equivalent to 240 nmol/min/mg as per radiometric assay
  • SDS PAGE analysis of ab135222
  • SDS PAGE analysis of ab135222
  • The specific activity of ab135222 was determined to be 242 nmol/min/mg.
  • SDS-PAGE analysis of ab135222.

References

ab135222 has not yet been referenced specifically in any publications.

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