The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.5 Constituent: 1.19% HEPES
A1S9T and BN75 temperature sensitivity complementing
POC20 centriolar protein homolog
UBA1, ubiquitin-activating enzyme E1 homolog A
Ubiquitin activating enzyme E1
Ubiquitin-activating enzyme E1
Ubiquitin-like modifier-activating enzyme 1
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
Protein modification; protein ubiquitination.
Involvement in disease
Defects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.