Recombinant Human E2F3 protein (ab114534)

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SDS-PAGE - Recombinant Human E2F3 protein (ab114534)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human E2F3 protein

    • Expression system

      Wheat germ

    • Accession

      O00716

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MQSGGGVKTDDTSTLNSLCGYAWVYVWEEKQRCRLSSFFSSSASIPGLLP SHTLDLVQNVGVVLDEALGWGRERELCVKCLLEMHCGVFSCMGNHLCQAF PHFPYLSHLVSCLCFQLCVILFASCTKLIFSKV

      • Predicted molecular weight

        41 kDa including tags

      • Amino acids

        1 to 133

    Specifications

    Our Abpromise guarantee covers the use of ab114534 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      SDS-PAGE

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • DKFZp686C18211
      • E2F 3
      • E2F transcription factor 3
      • E2F-3
      • E2F3
      • E2F3_HUMAN
      • KIAA0075
      • MGC104598
      • Transcription factor E2F 3
      • Transcription factor E2F3
      see all

    • Function

      Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-3 binds specifically to RB1 protein, in a cell-cycle dependent manner.

    • Sequence similarities

      Belongs to the E2F/DP family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human E2F3 protein (ab114534)
      SDS-PAGE - Recombinant Human E2F3 protein (ab114534)
      12.5% SDS-PAGE showing ab114534 at approximately 40.74kDa stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab114534? Please let us know so that we can cite the reference in this datasheet.

    ab114534 has not yet been referenced specifically in any publications.

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