Recombinant Human E2F8 protein (ab153513)

Submit a review Submit a question

Description

  • Product name

    Recombinant Human E2F8 protein

  • Expression system

    Wheat germ

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MAQLAAICKMQLEEQSSESRQKVKVQLARSGPCKPVAPLDPPVNAEMELT APSLIQPLGMVPLIPSPLSSAVPLILPQAPSGPSYAIYLQPTQAHQSVTP PQGLSPTVCTTHSSKATGSKDSTDATTEKAANDTSKASASTRPGSLLPAP ERQGAKSRTREPAGERGSKRASMLEDSGSKKKFKEDLKGLENVSATLFPS GYLIPLTQCSSLGAESILSGKENSSALSPNHRIYSSPIAGVIPVTSSELT AVNFPSFHVTPLKLMVSPTSVAAVPVGNSPALASSHPVPIQNPSSAIVNF TLQHLGLISPNVQLSASPGSGIVPVSPRIESVNVAPENAGTQQGRATNYD SPVPGQSQPNGQSVAVTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSS SCMDFEGANKTSLGTLFVPQRKLEVSTEDVH

    • Amino acids

      1 to 431

    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab153513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form

    Liquid

  • Additional notes

     

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • E2F 8
    • E2F family member 82
    • E2F transcription factor 8
    • E2F-8
    • E2f8
    • E2F8_HUMAN
    • FLJ23311
    • Transcription factor E2F8
    see all

  • Function

    Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.

  • Sequence similarities

    Belongs to the E2F/DP family.

  • Domain

    Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human E2F8 protein (ab153513)
    SDS-PAGE - Recombinant Human E2F8 protein (ab153513)
    ab153513 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab153513? Please let us know so that we can cite the reference in this datasheet.

ab153513 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab153513.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com