Recombinant Human E3 SUMO-protein ligase PIAS4/PIASy (ab153357)

Overview

  • Product name
    Recombinant Human E3 SUMO-protein ligase PIAS4/PIASy
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MAAELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDC SPELFKKIKELYETRYAKKNSEPAPQPHRPLDPLTMHSTYDRAGAVPRTP LAGPNIDYPVLYGKYLNGLGRLPAKTLKPEVRLVKLPFFNMLDELLKPTE LVPQNNEKLQESPCIFALTPRQVELIRNSRELQPGVKAVQVVLRICYSDT SCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMY LSSATNRITVTWGNYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCK ALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDA VFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLV DGSWCPIRAEKERSCSPQGAILVLGPSDANGLLPAPSVNGSGALGSTGGG GPVGSMENGKPGADVVDLTLDSSSSSEDEEEGEEEEEDEDEEGPRPKRRC PFQKGLVPAC
    • Amino acids
      1 to 510
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab153357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • E3 SUMO protein ligase PIAS4
    • E3 SUMO-protein ligase PIAS4
    • FLJ12419
    • MGC35296
    • PAIASgamma
    • PIAS 4
    • PIAS gamma
    • PIAS-gamma
    • Pias4
    • PIAS4_HUMAN
    • PIASG
    • PIASgamma
    • PIASy
    • Protein inhibitor of activated STAT 4
    • Protein inhibitor of activated STAT protein 4
    • Protein inhibitor of activated STAT protein gamma
    • Protein inhibitor of activated STAT protein PIASy
    • Zinc finger MIZ type containing 6
    • ZMIZ 6
    • ZMIZ6
    see all
  • Function
    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.
  • Tissue specificity
    Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
  • Pathway
    Protein modification; protein sumoylation.
  • Sequence similarities
    Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • Domain
    The LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
  • Post-translational
    modifications
    Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activitation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
  • Cellular localization
    Nucleus > PML body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.
  • Information by UniProt

Images

  • ab153357 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab153357 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153357.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up