Description

  • Product name

    Recombinant Human ECRG-2 protein
  • Purity

    > 85 % SDS-PAGE.
    ab167899 is purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSSEAASLS PKKVDCSIYK KYPVVAIPCP ITYLPVCGSD YITYGNECHL CTESLKSNGR VQFLHDGSC
    • Predicted molecular weight

      10 kDa including tags
    • Amino acids

      20 to 85
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab167899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Additional notes

    Previously labelled as SPINK7. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.03% DTT, 0.32% Tris HCl, 40% Glycerol, 1.17% Sodium chloride

General Info

  • Alternative names

    • ECG2
    • ECRG-2
    • Esophagus cancer-related gene 2 protein
    • ISK7_HUMAN
    • Serine protease inhibitor Kazal-type 7
    • SPINK7
    • UNQ745/PRO1474
    see all
  • Function

    Probable serine protease inhibitor.
  • Sequence similarities

    Contains 1 Kazal-like domain.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab167899 (3µg).

References

ab167899 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Functional Studies
1 active unit/well of uPA was loaded into a 96 well plate containing assay buffer (0.1 M NaH2PO4, pH 8.0) with or without 2.5 ug of human recombinant SPINK7. The samples were loaded with a chromogenic uPA substrate (from uPA activity assay, Millipore. ECM600) and incubated for 45 min at 37C.
Then the OD (405 nm) was measured.
It seems like this recombinant protein is functional as a serine protease inhibitor and works great.

Abcam user community

Verified customer

Submitted Dec 08 2014

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