Recombinant Human EDC3 protein (ab132637)

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SDS-PAGE - Recombinant Human EDC3 protein (ab132637)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: SDS-PAGE, WB, ELISA

    Description

    • Product name

      Recombinant Human EDC3 protein

    • Expression system

      Wheat germ

    • Accession

      Q96F86

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MATDWLGSIVSINCGDSLGVYQGRVSAVDQVSQTISLTRPFHNGVKCLVP EVTFRAGDITELKILEIPGPGDNQHFGDLHQTELGPSGAGCQVGINQNGT GKFVKKPASSSSAPQNIPKRTDVKSQDVAVSPQQQQCSKSYVDRHMESLS QSKSFRRRHNSWSSSSRHPNQATPKKSGLKNGQMKNKDDECFGDDIEEIP DTDFDFEGNLALFDKAAVFEEIDTYERRSGTRSRGIPNERPTRYRHDENI LESEPIVYRRIIVPHNVSKEFCTDSGLVVPSISYELHKKLLSVAEKHGLT LERRLEMTGVCASQMALTLLGGPNRLNPKNVHQRPTVALLCGPHVKGAQG ISCGRHLANHDVQVILFLPNFVKMLESITNELSLFSKTQGQQVSSLKDLP TSPVDLVINCLDCPENVFLRDQPWYKAAVAWANQNRAPVLSIDPPVHEVE QGIDAKWSLALGLPLPLGEHAGRIYLCDIGIPQQVFQEVGINYHSPFGCK FVIPLHSA

      • Predicted molecular weight

        82 kDa including tags

      • Amino acids

        1 to 508

    Specifications

    Our Abpromise guarantee covers the use of ab132637 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Western blot

      ELISA

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • EDC 3
      • edc3
      • EDC3_HUMAN
      • Enhancer of mRNA decapping 3 homolog
      • enhancer of mRNA decapping 3 homolog (S. cerevisiae)
      • Enhancer of mRNA decapping 3, S. cerevisiae, homolog of
      • Enhancer of mRNA decapping protein 3
      • Enhancer of mRNA-decapping protein 3
      • FLJ21128
      • FLJ31777
      • hYjeF_N2
      • hYjeF_N2-15q23
      • LSM 16
      • LSM16
      • LSM16 homolog
      • LSM16 homolog (EDC3 S. cerevisiae)
      • LSM16 homolog (EDC3, S. cerevisiae)
      • YJDC
      • YjeF domain containing
      • YjeF domain containing protein
      • YjeF domain containing protein 1
      • YjeF domain-containing protein 1
      • YjeF N-terminal domain-containing protein 2
      • YjeF_N2
      • YJEFN2
      see all

    • Function

      Binds single-stranded RNA. In the process of mRNA degradation, may play a role in mRNA decapping. May play a role in spermiogenesis and oogenesis.

    • Tissue specificity

      Expressed in theca and granulosa cells in ovary, and in spermatids of the meiotic division part II and apical membrane of Sertoli cells in testis (at protein level). Also expressed in brain and mammary gland.

    • Sequence similarities

      Belongs to the EDC3 family.
      Contains 1 DFDF domain.
      Contains 1 YjeF N-terminal domain.

    • Domain

      The DFDF domain is unstructured by itself. It assumes a helical fold upon interaction with DDX6.

    • Cellular localization

      Cytoplasm > P-body. Processing bodies.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human EDC3 protein (ab132637)
      SDS-PAGE - Recombinant Human EDC3 protein (ab132637)
      12.5% SDS-PAGE analysis of ab132637 stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab132637? Please let us know so that we can cite the reference in this datasheet.

    ab132637 has not yet been referenced specifically in any publications.

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