Recombinant Human eEF1A1/EF-Tu protein (ab81792)
Key features and details
- Expression system: Escherichia coli
- Tags: proprietary tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human eEF1A1/EF-Tu protein
See all eEF1A1/EF-Tu proteins and peptides -
Purity
90 % Ion Exchange Chromatography.
ab81792 is purified by an affinity column in combination with FPLC chromatography. Purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity. 1 unit equals 1 nanogram of purified protein. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMG KGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIK NMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIV GVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDN MLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPL QDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALS EALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHP GQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAA IVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGK VTKSAQKAQKAK -
Predicted molecular weight
76 kDa including tags -
Amino acids
1 to 462 -
Tags
proprietary tag N-Terminus -
Additional sequence information
RRASVNSH = the linker region between the tag at the N terminus and the protein sequence.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab81792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.9
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- CCS 3
- CCS3
- Cervical cancer suppressor 3
see all -
Function
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. -
Tissue specificity
Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab81792 has not yet been referenced specifically in any publications.