Recombinant Human EIF3F protein (denatured) (ab171467)
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- References
- Protocols
Description
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Product name
Recombinant Human EIF3F protein (denatured) -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMATPAVP VSAPPATPTP VPAAAPASVP APTPAPAAAP VPAAAPASSS DPAAAAAATA APGQTPASAQ APAQTPAPAL PGPALPGPFP GGRVVRLHPV ILASIVDSYE RRNEGAARVI GTLLGTVDKH SVEVTNCFSV PHNESEDEVA VDMEFAKNMY ELHKKVSPNE LILGWYATGH DITEHSVLIH EYYSREAPNP IHLTVDTSLQ NGRMSIKAYV STLMGVPGRT MGVMFTPLTV KYAYYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVPDDFETML NSNINDLLMV TYLANLTQSQ IALNEKLVNL -
Predicted molecular weight
40 kDa including tags -
Amino acids
1 to 357 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab171467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 2.4% Urea, 0.32% Tris, 10% Glycerol
General Info
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Alternative names
- Deubiquitinating enzyme eIF3f
- eIF 3 epsilon
- eIF-3-epsilon
see all -
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling. -
Sequence similarities
Belongs to the eIF-3 subunit F family.
Contains 1 MPN (JAB/Mov34) domain. -
Domain
The MPN domain mediates deubiquitinating activity. -
Post-translational
modificationsPhosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human EIF3F protein (denatured) (ab171467)15% SDS-PAGE analysis of ab171467 (3µg).
Datasheets and documents
References
ab171467 has not yet been referenced specifically in any publications.
