Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant Human eIF4E protein
See all eIF4E proteins and peptides
Purity> 90 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight51 kDa including tags
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab56276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
- eIF 4E
FunctionIts translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Sequence similaritiesBelongs to the eukaryotic initiation factor 4E family.
modificationsPhosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab56276 has been referenced in 4 publications.
- Wollenhaupt K et al. The eIF4E repressor protein 4E-BP2 is merely truncated, despite 4E-BP1 degradation in the porcine uterine tissue during implantation. Mol Reprod Dev 79:767-76 (2012). PubMed: 22968905
- Wollenhaupt K et al. 4E-BP1 degradation and eIF4E truncation occur spatially distinctly in the porcine uterine epithelia and are features of noninvasive implantation in the pig. Mol Reprod Dev 78:895-905 (2011). PubMed: 21887718
- Wollenhaupt K et al. Natural occurrence and physiological role of a truncated eIF4E in the porcine endometrium during implantation. Biochem J 432:353-63 (2010). PubMed: 20854261
- Siemer C et al. Analysis of mRNA associated factors during bovine oocyte maturation and early embryonic development. Mol Reprod Dev 76:1208-19 (2009). PubMed: 19697362