Recombinant Human eIF4EBP1 protein (Tagged) (ab85248)
Key features and details
- Expression system: Escherichia coli
- Purity: > 75% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB
Description
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Product name
Recombinant Human eIF4EBP1 protein (Tagged)
See all eIF4EBP1 proteins and peptides -
Purity
> 75 % Densitometry. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Molecular weight information
Approx 40 kDa by SDS-PAGE -
Tags
GST tag N-Terminus -
Additional sequence information
GenBank: NM_004095
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab85248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
General Info
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Alternative names
- 4E-BP1
- 4EBP1
- 4EBP1_HUMAN
see all -
Function
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. -
Sequence similarities
Belongs to the eIF4E-binding protein family. -
Post-translational
modificationsPhosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85248 has not yet been referenced specifically in any publications.