Key features and details
- Expression system: Escherichia coli
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB
Product nameRecombinant Human eIF4EBP1 protein (Tagged)
See all eIF4EBP1 proteins and peptides
Expression systemEscherichia coli
Protein lengthFull length protein
Molecular weight informationApprox 40 kDa by SDS-PAGE
TagsGST tag N-Terminus
Additional sequence informationGenBank: NM_004095
Our Abpromise guarantee covers the use of ab85248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
FunctionRegulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Sequence similaritiesBelongs to the eIF4E-binding protein family.
modificationsPhosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab85248 has not yet been referenced specifically in any publications.