Overview

Description

  • Nature

    Recombinant
  • Source

    Wheat germ
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MATAETALPSISTLTALGPFPDTQDDFLKWWRSEEAQDMGPGPPDPTEPP LHVKSEDQPGEEEDDERGADATWDLDLLLTNFSGPEPGGAPQTCALAPSE APGAQYPPPPETLGAYAGGPGLVAGLLGSEDHSGWVRPALRARAPDAFVG PALAPAPAPEPKALALQPVYPGPGAGSSGGYFPRTGLSVPAASGAPYGLL SGYPAMYPAPQYQGHFQLFRGLQGPAPGPATSPSFLSCLGPGTVGTGLGG TAEDPGVIAETAPSKRGRRSWARKRQAAHTCAHPGCGKSYTKSSHLKAHL RTHTGEKPYACTWEGCGWRFARSDELTRHYRKHTGQRPFRCQLCPRAFSR SDHLALHMKRHL
    • Molecular weight

      65 kDa including tags
    • Amino acids

      1 to 362

Specifications

Our Abpromise guarantee covers the use of ab131863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Additional notes

    Protein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • CDAN4
    • EKLF
    • EKLF PEN
    • Erythroid krueppel like transcription factor (EKLF) (Erythroid transcription factor)
    • Erythroid krueppel-like transcription factor
    • Erythroid Kruppel like factor
    • Erythroid specific transcription factor EKLF
    • HBFQTL6
    • Human erythroid-specific transcription factor EKLF mRNA complete cds
    • INLU
    • Klf1
    • KLF1_HUMAN
    • Krueppel-like factor 1
    • Kruppel like factor 1 (erythroid)
    see all
  • Function

    Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.
  • Tissue specificity

    Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.
  • Involvement in disease

    Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4) [MIM:613673]. It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.
    Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.
    Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.
  • Cellular localization

    Nucleus. Colocalizes with SUMO1 in nuclear speckles.
  • Information by UniProt

Images

  • 12.5% SDS-PAGE analysis of ab131863 stained with Coomassie Blue.

References

ab131863 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Unfortunately, we have not determined ab131863's conformation yet. Therefore, we are not sure if the folding of this protein is correct.

The only applications in which we have tested this protein in are ELISA, SDS-PAGE and Western blot which are not helpful in determining the folding of the protein.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up