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    recombinant-human-emap-iiaimp1-protein-ab199808.pdf

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Cell Biology Apoptosis Intracellular Kinases
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Bioactive grade

Recombinant human EMAP II/AIMP1 protein (ab199808)

  • Datasheet
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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Suitable for: Functional Studies, HPLC, SDS-PAGE

Description

  • Product name

    Recombinant human EMAP II/AIMP1 protein
    See all EMAP II/AIMP1 proteins and peptides
  • Biological activity

    Determined by the apoptotic effect on MCF-7 cells using a concentration of 20-40 ng/mL.

  • Purity

    > 98 % SDS-PAGE.
    Purity determined by SDS-PAGE and HPLC analyses.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

    Q12904
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK
    • Predicted molecular weight

      18 kDa
    • Amino acids

      147 to 312
    • Additional sequence information

      This is the full length Endothelial monocyte-activating polypeptide 2 amino acids 147-312.

Specifications

Our Abpromise guarantee covers the use of ab199808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    HPLC

    SDS-PAGE

  • Form

    Lyophilized
  • Additional notes

     This product was previously labelled as EMAP II

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 0.75% Sodium chloride, 99% Phosphate Buffer

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    The recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

General Info

  • Alternative names

    • AIMP 1
    • Aimp1
    • AIMP1/p43
    • AIMP1_HUMAN
    • Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
    • ARS interacting multifunctional protein 1
    • EMAP 2
    • EMAP-2
    • EMAP-II
    • EMAP2
    • EMAPII
    • Endothelial monocyte activating polypeptide
    • Endothelial monocyte activating polypeptide 2
    • Endothelial monocyte-activating polypeptide 2
    • Endothelial monocyte-activating polypeptide II
    • HLD3
    • Multisynthase complex auxiliary component p43
    • Multisynthetase complex auxiliary component p43
    • OTTHUMP00000219673
    • p43
    • Scye 1
    • Scye1
    • Scye1, formerly
    • Small inducible cytokine subfamily E member 1
    • Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
    • Small inducible cytokine subfamily E, member 1, formerly
    see all
  • Function

    Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
  • Involvement in disease

    Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3) [MIM:260600]. A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.
  • Sequence similarities

    Contains 1 tRNA-binding domain.
  • Post-translational
    modifications

    Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.
  • Cellular localization

    Nucleus. Cytoplasm > cytosol. Cytoplasmic vesicle > secretory vesicle. Secreted. Endoplasmic reticulum. Golgi apparatus. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death.
  • Target information above from: UniProt accession Q12904 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab199808? Please let us know so that we can cite the reference in this datasheet.

    ab199808 has not yet been referenced specifically in any publications.

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