Recombinant Human Emerin protein (ab112283)
Key features and details
- Expression system: Wheat germ
- Suitable for: WB, ELISA, PepArr, SDS-PAGE
Description
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Product name
Recombinant Human Emerin protein -
Biological activity
useful for Antibody Production and Protein Array -
Expression system
Wheat germ -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
54 kDa including tags -
Amino acids
1 to 254
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab112283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
ELISA
Peptide Array
SDS-PAGE
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Form
Liquid -
Additional notes
This product is useful for Antibody Production and Protein Array.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
Reduced glutathione
General Info
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Alternative names
- EDMD
- Emd
- EMD_HUMAN
see all -
Function
Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C. -
Tissue specificity
Skeletal muscle, heart, colon, testis, ovary and pancreas. -
Involvement in disease
Defects in EMD are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1) [MIM:310300]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. -
Sequence similarities
Contains 1 LEM domain. -
Post-translational
modificationsFound in four different phosphorylated forms, three of which appear to be associated with the cell cycle. -
Cellular localization
Nucleus inner membrane. Nucleus outer membrane. Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab112283 has not yet been referenced specifically in any publications.