Overview

  • Product name
    Recombinant Human EMG1 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      Q92979
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MGSMAAPSDG FKPRERSGGE QAQDWDALPP KRPRLGAGNK IGGRRLIVVL EGASLETVKV GKTYELLNCD KHKSILLKNG RDPGEARPDI THQSLLMLMD SPLNRAGLLQ VYIHTQKNVL IEVNPQTRIP RTFDRFCGLM VQLLHKLSVR AADGPQKLLK VIKNPVSDHF PVGCMKVGTS FSIPVVSDVR ELVPSSDPIV FVVGAFAHGK VSVEYTEKMV SISNYPLSAA LTCAKLTTAF EEVWGVI
    • Molecular weight
      29 kDa including tags
    • Amino acids
      1 to 244
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab130059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    >95% by SDS-PAGE .
    ab130059 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names
    • 18S rRNA (pseudouridine(1248)-N1)-methyltransferase
    • 18S rRNA Psi1248 methyltransferase
    • C2F
    • C2f protein
    • EMG 1
    • EMG1
    • EMG1 N1 specific pseudouridine methyltransferase
    • EMG1 nucleolar protein homolog
    • EMG1 nucleolar protein homolog (S cerevisiae)
    • essential for mitotic growth 1
    • FLJ60792
    • Grcc2f
    • NEP1
    • NEP1_HUMAN
    • Nucleolar protein EMG1 homolog
    • Protein C2f
    • Ribosomal RNA small subunit methyltransferase Nep1
    • Ribosome biogenesis protein NEP1
    see all
  • Function
    S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed:20047967). Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes.
  • Involvement in disease
    Bowen-Conradi syndrome
  • Sequence similarities
    Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.
  • Cellular localization
    Nucleus, nucleolus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human EMG1 protein (ab130059)
    SDS-PAGE - Recombinant Human EMG1 protein (ab130059)
    15% SDS-PAGE showing ab130059 (3µg).

References

ab130059 has not yet been referenced specifically in any publications.

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