Recombinant Human EMG1 protein (ab130059)

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SDS-PAGE - Recombinant Human EMG1 protein (ab130059)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human EMG1 protein

    • Purity

      > 95 % SDS-PAGE.
      ab130059 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      Q92979

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSMAAPSDG FKPRERSGGE QAQDWDALPP KRPRLGAGNK IGGRRLIVVL EGASLETVKV GKTYELLNCD KHKSILLKNG RDPGEARPDI THQSLLMLMD SPLNRAGLLQ VYIHTQKNVL IEVNPQTRIP RTFDRFCGLM VQLLHKLSVR AADGPQKLLK VIKNPVSDHF PVGCMKVGTS FSIPVVSDVR ELVPSSDPIV FVVGAFAHGK VSVEYTEKMV SISNYPLSAA LTCAKLTTAF EEVWGVI

      • Predicted molecular weight

        29 kDa including tags

      • Amino acids

        1 to 244

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab130059 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    General Info

    • Alternative names

      • 18S rRNA (pseudouridine(1248)-N1)-methyltransferase
      • 18S rRNA Psi1248 methyltransferase
      • C2F
      • C2f protein
      • EMG 1
      • EMG1
      • EMG1 N1 specific pseudouridine methyltransferase
      • EMG1 nucleolar protein homolog
      • EMG1 nucleolar protein homolog (S cerevisiae)
      • essential for mitotic growth 1
      • FLJ60792
      • Grcc2f
      • NEP1
      • NEP1_HUMAN
      • Nucleolar protein EMG1 homolog
      • Protein C2f
      • Ribosomal RNA small subunit methyltransferase Nep1
      • Ribosome biogenesis protein NEP1
      see all

    • Function

      S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed:20047967). Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes.

    • Involvement in disease

      Bowen-Conradi syndrome

    • Sequence similarities

      Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.

    • Cellular localization

      Nucleus, nucleolus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human EMG1 protein (ab130059)
      SDS-PAGE - Recombinant Human EMG1 protein (ab130059)
      15% SDS-PAGE showing ab130059 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab130059? Please let us know so that we can cite the reference in this datasheet.

    ab130059 has not yet been referenced specifically in any publications.

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