Recombinant human Endostatin/COL18A1 protein (ab56290)

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Western blot - Recombinant human Endostatin/COL18A1 protein (ab56290)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 0.100 Eu/µg
    • Active: Yes
    • Suitable for: SDS-PAGE, Functional Studies, WB

    Description

    • Product name

      Recombinant human Endostatin/COL18A1 protein
      See all Endostatin/COL18A1 proteins and peptides

    • Purity

      > 95 % SDS-PAGE.
      Purity : Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).

    • Endotoxin level

      < 0.100 Eu/µg

    • Expression system

      Escherichia coli

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MHSHRDFQPV LHLVALNSPL SGGMRGIRGA DFQCFQQARA VGLAGTFRAF LSSRLQDLYS IVRRADRAAV PIVNLKDELL FPSWEALFSG SEGPLKPGAR IFSFDGKDVL RHPTWPQKSV WHGSDPNGRR LTESYCETWR TEAPSATGQA SSLLGGRLLG QSAASCHHAY IVLCIENSFM TASK

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab56290 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

      Western blot

    • Form

      Lyophilized

    • Additional notes

       This product was previously labelled as Endostatin

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      For lot specific reconstitution information please contact our Scientific Support Team.

    General Info

    • Alternative names

      • Alpha 1 collagen type 18 (XVIII)(COL18A1)
      • Alpha 1 type XVIII collagen
      • Antiangiogenic agent
      • COIA1_HUMAN
      • COL15A1
      • Col18a1
      • Collagen alpha 1(XV) chain
      • Collagen alpha 1(XVIII) chain
      • Collagen alpha-1(XV) chain
      • Collagen type XV proteoglycan
      • Collagen type XVIII alpha 1
      • Collagen XV, alpha 1 polypeptide
      • Collagen, type XV, alpha 1
      • Endostatin
      • Endostatin XV
      • FLJ27325
      • FLJ34914
      • FLJ38566
      • KNO
      • KNO1
      • KS
      • MGC74745
      • Multi functional protein MFP
      • OTTHUMP00000021782
      • OTTHUMP00000115472
      • OTTHUMP00000115473
      see all

    • Function

      COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
      Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.

    • Tissue specificity

      Present in multiple organs with highest levels in liver, lung and kidney.

    • Involvement in disease

      Defects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele.

    • Sequence similarities

      Belongs to the multiplexin collagen family.
      Contains 1 FZ (frizzled) domain.
      Contains 1 TSP N-terminal (TSPN) domain.

    • Post-translational
      modifications

      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt

    Images

    • Western blot - Recombinant human Endostatin/COL18A1 protein (ab56290)
      Western blot - Recombinant human Endostatin/COL18A1 protein (ab56290)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab56290? Please let us know so that we can cite the reference in this datasheet.

    ab56290 has not yet been referenced specifically in any publications.

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