Recombinant human ENO1 protein (Active) (ab89248)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, WB, SDS-PAGE
Description
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Product name
Recombinant human ENO1 protein (Active)
See all ENO1 proteins and peptides -
Biological activity
Specific activity is > 20,000pmol/min/ug.
Obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.
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Purity
> 90 % SDS-PAGE.
ab89248 is purified using conventional chromatography techniques. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK -
Predicted molecular weight
47 kDa -
Amino acids
1 to 434 -
Additional sequence information
NCBI: NP_001419
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab89248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
Western blot
SDS-PAGE
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Form
Liquid -
Additional notes
MW confirmed by MALDI-TOF.
Endotoxin level determined by LAL method.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.012% Magnesium sulphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 2 phospho D glycerate hydro lyase
- 2-phospho-D-glycerate hydro-lyase
- Alpha enolase
see all -
Function
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Post-translational
modificationsISGylated. -
Cellular localization
Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human ENO1 protein (ab89248)
3 ug of reduced ab89248 on 15% SDS-PAGE, stained by Coomassie Blue.
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Western blot - Recombinant Human ENO1 protein (ab89248)Anti-ENO1 antibody (ab85086) at 1/1000 dilution +Recombinant human ENO1 protein (Active) (ab89248) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Exposure time: 30 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab89248 has been referenced in 1 publication.
- Higa LM et al. Modulation of a-Enolase Post-Translational Modifications by Dengue Virus: Increased Secretion of the Basic Isoforms in Infected Hepatic Cells. PLoS One 9:e88314 (2014). ELISA . PubMed: 25171719
