Bioactive grade

Recombinant human ENO1 protein (Active) (ab89248)

Submit a review Q&A (1)References (1)

Description

  • Product name

    Recombinant human ENO1 protein (Active)
    See all ENO1 proteins and peptides

  • Biological activity

    Specific activity is > 20,000pmol/min/ug.

    Obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.

  • Purity

    > 90 % SDS-PAGE.
    ab89248 is purified using conventional chromatography techniques.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P06733

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK

    • Predicted molecular weight

      47 kDa

    • Amino acids

      1 to 434

    • Additional sequence information

      NCBI: NP_001419

Associated products

Specifications

Our Abpromise guarantee covers the use of ab89248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    Western blot

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    MW confirmed by MALDI-TOF.

    Endotoxin level determined by LAL method.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.316% Tris HCl, 10% Glycerol, 0.012% Magnesium sulphate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 2 phospho D glycerate hydro lyase
    • 2-phospho-D-glycerate hydro-lyase
    • Alpha enolase
    • Alpha enolase like 1
    • Alpha-enolase
    • C myc promoter binding protein
    • C-myc promoter-binding protein
    • EC 4.2.1.11
    • eno1
    • ENO1L1
    • ENOA_HUMAN
    • Enolase 1
    • Enolase 1 (alpha)
    • Enolase 1 (alpha) like 1
    • Enolase alpha
    • MBP 1
    • MBP-1
    • MBP1
    • MBPB1
    • MPB 1
    • MPB-1
    • MPB1
    • MYC promoter binding protein 1
    • NNE
    • Non neural enolase
    • Non-neural enolase
    • Phosphopyruvate hydratase
    • Plasminogen binding protein
    • Plasminogen-binding protein
    • PPH
    • Tau crystallin
    see all

  • Function

    Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

  • Sequence similarities

    Belongs to the enolase family.

  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

  • Post-translational
    modifications

    ISGylated.

  • Cellular localization

    Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human ENO1 protein (ab89248)
    SDS-PAGE - Recombinant Human ENO1 protein (ab89248)

    3 ug of reduced ab89248 on 15% SDS-PAGE, stained by Coomassie Blue.

  • Western blot - Recombinant Human ENO1 protein (ab89248)
    Western blot - Recombinant Human ENO1 protein (ab89248)
    Anti-ENO1 antibody (ab85086) at 1/1000 dilution + Recombinant human ENO1 protein (Active) (ab89248) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa


    Exposure time: 30 seconds

References

This product has been referenced in:

  • Higa LM  et al. Modulation of a-Enolase Post-Translational Modifications by Dengue Virus: Increased Secretion of the Basic Isoforms in Infected Hepatic Cells. PLoS One 9:e88314 (2014). ELISA . Read more (PubMed: 25171719) »
See 1 Publication for this product

Publishing research using ab89248? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Recently, I bought a vial of the recombinant enolase 1 protein (ab89248) but I lost the datasheet. So I would like to ask for the datasheet and any other information on this product.  

Read More
Answer

Thank you for contacting us. Attached is the requested datasheet. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com