Recombinant Human ENO3 protein (ab113127)

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Description

  • Product name

    Recombinant Human ENO3 protein

  • Biological activity

    Specific activity is > 5,000pmol/min/µg, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37°C.

  • Purity

    > 95 % SDS-PAGE.
    ab113127 was purified using conventional chromatography.

  • Expression system

    Escherichia coli

  • Accession

    P13929

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMAMQKIFAREILDSRGNPTVEVDLHTAKGR FRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINSTLGPALLQK KLSVADQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPL YRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKE AMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTA IQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELY KSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQ AVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTF IADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRN PKAK

    • Predicted molecular weight

      49 kDa including tags

    • Amino acids

      1 to 434

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab113127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 2 phospho D glycerate hydro lyase
    • 2 phospho D glycerate hydrolyase
    • 2-phospho-D-glycerate hydro-lyase
    • Beta enolase
    • Beta-enolase
    • ENO3
    • ENOB_HUMAN
    • Enolase 3
    • Enolase 3 (beta muscle)
    • Enolase3
    • GSD13
    • MSE
    • Muscle specific enolase
    • Muscle-specific enolase
    • Skeletal muscle enolase
    see all

  • Function

    Appears to have a function in striated muscle development and regeneration.

  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

  • Involvement in disease

    Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13) [MIM:612932]. A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen-beta particles are detected on ultrastructural analysis.

  • Sequence similarities

    Belongs to the enolase family.

  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

  • Cellular localization

    Cytoplasm. Localized to the Z line. Some colocalization with CKM at M-band.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human ENO3 protein (ab113127)
    SDS-PAGE - Recombinant Human ENO3 protein (ab113127)
    15% SDS-PAGE analysis of ENO3 protein (ab113127; 3 µg).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab113127? Please let us know so that we can cite the reference in this datasheet.

ab113127 has not yet been referenced specifically in any publications.

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