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    recombinant-human-enpp1pc1-protein-ab167943.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Bone
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Recombinant Human ENPP1/PC1 protein (ab167943)

  • Datasheet
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SDS-PAGE - Recombinant Human ENPP1/PC1 protein (ab167943)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 95% Densitometry
    • Endotoxin level: < 1.000 Eu/µg
    • Tags: His tag C-Terminus
    • Suitable for: WB, ELISA, SDS-PAGE

    Description

    • Product name

      Recombinant Human ENPP1/PC1 protein
    • Purity

      > 95 % Densitometry.
      ab167943 was purifed using Ni-NTA chromatography.
    • Endotoxin level

      < 1.000 Eu/µg
    • Expression system

      HEK 293 cells
    • Accession

      P22413
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        ASKPSCAKEV KSCKGRCFER TFGNCRCDAA CVELGNCCLD YQETCIEPEH IWTCNKFRCG EKRLTRSLCA CSDDCKDKGD CCINYSSVCQ GEKSWVEEPC ESINEPQCPA GFETPPTLLF SLDGFRAEYL HTWGGLLPVI SKLKKCGTYT KNMRPVYPTK TFPNHYSIVT GLYPESHGII DNKMYDPKMN ASFSLKSKEK FNPEWYKGEP IWVTAKYQGL KSGTFFWPGS DVEINGIFPD IYKMYNGSVP FEERILAVLQ WLQLPKDERP HFYTLYLEEP DSSGHSYGPV SSEVIKALQR VDGMVGMLMD GLKELNLHRC LNLILISDHG MEQGSCKKYI YLNKYLGDVK NIKVIYGPAA RLRPSDVPDK YYSFNYEGIA RNLSCREPNQ HFKPYLKHFL PKRLHFAKSD RIEPLTFYLD PQWQLALNPS ERKYCGSGFH GSDNVFSNMQ ALFVGYGPGF KHGIEADTFE NIEVYNLMCD LLNLTPAPNN GTHGSLNHLL KNPVYTPKHP KEVHPLVQCP FTRNPRDNLG CSCNPSILPI EDFQTQFNLT VAEEKIIKHE TLPYGRPRVL QKENTICLLS QHQFMSGYSQ DILMPLWTSY TVDRNDSFST EDFSNCLYQD FRIPLSPVHK CSFYKNNTKV SYGFLSPPQL NKNSSGIYSE ALLTTNIVPM YQSFQVIWRY FHDTLLRKYA EERNGVNVVS GPVFDFDYDG RCDSLENLRQ KRRVIRNQEI LIPTHFFIVL TSCKDTSQTP LHCENLDTLA FILPHRTDNS ESCVHGKHDS SWVEELLMLH RARITDVEHI TGLSFYQQRK EPVSDILKLK THLPTFSQED GPKLHHHHHH
      • Predicted molecular weight

        97 kDa including tags
      • Amino acids

        98 to 925
      • Tags

        His tag C-Terminus

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-ENPP1/PC1 antibody (ab40003)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab167943 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      ELISA

      SDS-PAGE

    • Form

      Lyophilized
    • Additional notes

       This product was previously labelled as ENPP1

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -80°C.

      Constituents: 99% Phosphate Buffer, 0.43% Sodium chloride

    • Reconstitution
      Add 200µl deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage.

      Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

    General Info

    • Alternative names

      • Alkaline phosphodiesterase 1
      • ARHR2
      • COLED
      • E-NPP 1
      • Ectonucleotide pyrophosphatase/phosphodiesterase 1
      • Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
      • ENPP1
      • ENPP1_HUMAN
      • Ly 41 antigen
      • M6S1
      • Membrane component chromosome 6 surface marker 1
      • NPP1
      • NPPase
      • NPPS
      • Nucleotide pyrophosphatase
      • PC 1
      • PC-1
      • PCA1
      • PDNP1
      • Phosphodiesterase I/nucleotide pyrophosphatase 1
      • Plasma cell membrane glycoprotein 1
      • Plasma-cell membrane glycoprotein PC-1
      see all
    • Function

      Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity.
    • Tissue specificity

      Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis.
    • Involvement in disease

      Defects in ENPP1 are a cause of increased susceptibility for ossification of the posterior longitudinal ligament of the spine (OPLL) [MIM:602475]. OPLL is a common form of human myelopathy with a prevalence of as much as 4% in a variety of ethnic groups.
      Defects in ENPP1 are the cause of arterial calcification of infancy, generalized, type 1 (GACI1) [MIM:208000]. A severe autosomal recessive disorder characterized by calcification of the internal elastic lamina of muscular arteries and stenosis due to myointimal proliferation. The disorder is often fatal within the first 6 months of life because of myocardial ischemia resulting in refractory heart failure.
      Defects in ENPP1 are associated with obesity, glucose intolerance, and type II diabetes non-insulin dependent (NIDDM) [MIM:125853].
      Defects in ENPP1 are the cause of rickets hypophosphatemic autosomal recessive type 2 (ARHR2) [MIM:613312]. ARHR2 is a hereditary form of hypophosphatemic rickets, a disorder of proximal renal tubule function that causes phosphate loss, hypophosphatemia and skeletal deformities, including rickets and osteomalacia unresponsive to vitamin D. Symptoms are bone pain, fractures and growth abnormalities.
    • Sequence similarities

      Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
      Contains 2 SMB (somatomedin-B) domains.
    • Domain

      The di-leucine motif is required for basolateral targeting in epithelial cells, and for targeting to matrix vesicles derived from mineralizing cells.
    • Post-translational
      modifications

      Autophosphorylated as part of the catalytic cycle of phosphodiesterase/pyrophosphatase activity.
      N-glycosylated.
      It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    • Cellular localization

      Membrane. Basolateral cell membrane. Targeted to the basolateral membrane in polarized epithelial cells and in hepatocytes, and to matrix vesicles in osteoblasts. In bile duct cells and cancer cells, located to the apical cytoplasmic side.
    • Target information above from: UniProt accession P22413 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human ENPP1/PC1 protein (ab167943)
      SDS-PAGE - Recombinant Human ENPP1/PC1 protein (ab167943)
      10% SDS-PAGE analysis of ab167943.
      Lane 1: reduced and boiled sample, 2.5μg/lane.
      Lane 2: non-reduced and non-boiled sample, 2.5μg/lane.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab167943? Please let us know so that we can cite the reference in this datasheet.

    ab167943 has been referenced in 1 publication.

    • Butler TAJ  et al. Misleading Westerns: Common Quantification Mistakes in Western Blot Densitometry and Proposed Corrective Measures. Biomed Res Int 2019:5214821 (2019). PubMed: 30800670

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