Bioactive grade

Recombinant human Eotaxin protein (Active) (ab243753)

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SDS-PAGE - Recombinant human Eotaxin protein (Active) (ab243753)

    Key features and details

    • Expression system: Escherichia coli
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Suitable for: Functional Studies, HPLC, SDS-PAGE

    Description

    • Product name

      Recombinant human Eotaxin protein (Active)
      See all Eotaxin proteins and peptides

    • Biological activity

      Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/ml.

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      Escherichia coli

    • Accession

      P51671

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        GPASVPTTCCFNLANRKIPLQRLESYRRITSGKCPQKAVIFKTKLAKDIC ADPKKKWVQDSMKYLDQKSPTPKP

      • Predicted molecular weight

        8 kDa

      • Amino acids

        24 to 97

      • Additional sequence information

        Full-length mature chain lacking the signal peptide.

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab243753 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      HPLC

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituent: PBS

      0.2 µm filtered

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at =-20 °C. Further dilutions should be made in appropriate buffered solutions.

    General Info

    • Alternative names

      • C-C motif chemokine 11
      • CCL 11
      • CCL11
      • CCL11_HUMAN
      • chemokine (C-C motif) ligand 11
      • Chemokine C C motif ligand 11
      • Chemokine CC Motif Ligand 11
      • Chemokine ligand 11
      • Eosinophil chemotactic protein
      • Eotaxin
      • eotaxin-1
      • MGC22554
      • SCYA 11
      • SCYA11
      • Small inducible cytokine A11
      • Small inducible cytokine subfamily A (Cys Cys) member 11
      • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
      • Small inducible cytokine subfamily A member 11
      • Small-inducible cytokine A11
      see all

    • Function

      In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.

    • Sequence similarities

      Belongs to the intercrine beta (chemokine CC) family.

    • Post-translational
      modifications

      O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues.

    • Cellular localization

      Secreted.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human Eotaxin protein (Active) (ab243753)
      SDS-PAGE - Recombinant human Eotaxin protein (Active) (ab243753)
      SDS-PAGE analysis of ab243753

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab243753? Please let us know so that we can cite the reference in this datasheet.

    ab243753 has been referenced in 1 publication.

    • Wang R & Huang K CCL11 increases the proportion of CD4+CD25+Foxp3+ Treg cells and the production of IL-2 and TGF-ß by CD4+ T cells via the STAT5 signaling pathway. Mol Med Rep 21:2522-2532 (2020). PubMed: 32323817

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