Overview

Description

  • Nature

    Recombinant
  • Source

    Wheat germ
  • Amino Acid Sequence
    • Species

      Human
    • Sequence

      FMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHNPVPL SPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWAYVQQLKVIDNQRE LSRLSRELEP
    • Amino acids

      772 to 881
    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab160976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

  • Form

    Liquid
  • Additional notes

    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • bcm910
    • CAMP GEFI
    • cAMP regulated guanine nucleotide exchange factor I
    • CAMPGEFI
    • CGEF 1
    • CGEF1
    • EPA1
    • EPAC
    • Epac 1
    • EPAC1
    • Exchange factor directly activated by cAMP 1
    • Exchange protein directly activated by cAMP 1
    • MGC21410
    • RAP guanine nucleotide exchange factor
    • Rap guanine nucleotide exchange factor (GEF) 3
    • RAP guanine nucleotide exchange factor 3
    • Rap1 guanine nucleotide exchange factor directly activated by cAMP
    • RAPGEF3
    see all
  • Relevance

    The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective antibodies were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The antibodies to Epac 1are affinity purified over immobilized antigen based chromatography.
  • Cellular localization

    Endomembrane system

Images

  • ab160976 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab160976 has not yet been referenced specifically in any publications.

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