The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
cAMP regulated guanine nucleotide exchange factor II
cAMP-regulated guanine nucleotide exchange factor II
Exchange factor directly activated by cAMP 2
Exchange protein directly activated by cAMP 2
Putative protein product of Nbla00496
Rap guanine nucleotide exchange factor (GEF) 4
Rap guanine nucleotide exchange factor 4
Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.
The N-terminal nucleotide phosphate binding region cAMP 1 has a much lower affinity for cAMP as compared to cAMP 2. The DEP domain is involved in membrane localization independent from regulation by cAMP.