Recombinant human Eph receptor A4/SEK protein (ab84575)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human Eph receptor A4/SEK protein
See all Eph receptor A4/SEK proteins and peptides -
Biological activity
Specific activity of Eph receptor A4/SEK was determined to be 83 nmol/min/mg as per activity assay protocol.
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Purity
> 90 % SDS-PAGE.
Purity was determined to be >90% by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Amino acids
610 to 887
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab84575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as Eph receptor A4
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Cek 8
- CEK8
- EK8
see all -
Function
Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in a signal transduction process involved in hindbrain pattern formation. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Domain
The protein kinase domain mediates interaction with NGEF/ephexin-1. -
Cellular localization
Membrane. - Information by UniProt
Images
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The specific activity of Eph receptor A4/SEK (ab84575) was determined to be 70 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab84575
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SDS PAGE analysis of ab84575
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Specific activity of Eph receptor A4/SEK was determined to be 83 nmol/min/mg as per activity assay protocol.
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Purity of Eph receptor A4/SEK was determined to be >90% by densitometry. approx, MW 58kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab84575 has not yet been referenced specifically in any publications.