Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human Eph receptor A4/SEK protein (Active)
See all Eph receptor A4/SEK proteins and peptides
Assay was done in kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 µM ATP. Reaction was performed at 30°C for 40 mins. Amount of ATP transferred was calculated using Kinase-Glo reagent.
Specific activity of 61 pmol/mon/µg.
Purity>= 90 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceREFAKEIDASCIKIEKVIGVGEFGEVCSGRLKVPGKREICVAIKTLKAGY TDKQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITEYMENGSLDA FLRKNDGRFTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLV CKVSDFGMSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWS YGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPPPMDCPIALHQLMLD CWQKERSDRPKFGQIVNMLDKLIRNPNS
Predicted molecular weight58 kDa
Amino acids610 to 887
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab271498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Cek 8
FunctionReceptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in a signal transduction process involved in hindbrain pattern formation.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
DomainThe protein kinase domain mediates interaction with NGEF/ephexin-1.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271498 has not yet been referenced specifically in any publications.